| Unique identifier for interactor A | uniprotkb:A2AIV8 |
| Unique identifier for interactor B | uniprotkb:Q9HC29 |
| Alternative identifier for interactor A | intact:EBI-9549098 ensembl:ENSMUSP00000028294.7 ensembl:ENSMUSP00000097876.4 |
| Alternative identifier for interactor B | intact:EBI-7445625 uniprotkb:Q96RH5 uniprotkb:Q96RH8 uniprotkb:Q96RH6 uniprotkb:E2JEQ6 intact:MINT-151071 ensembl:ENSP00000300589.2 |
| Aliases for A | psi-mi:card9_mouse(display_long) uniprotkb:Card9(gene name) psi-mi:Card9(display_short) |
| Aliases for B | psi-mi:nod2_human(display_long) uniprotkb:NOD2(gene name) psi-mi:NOD2(display_short) uniprotkb:CARD15(gene name synonym) uniprotkb:Caspase recruitment domain-containing protein 15(gene name synonym) uniprotkb:Inflammatory bowel disease protein 1(gene name synonym) |
| Interaction detection methods | psi-mi:"MI:0007"(anti tag coimmunoprecipitation) |
| First author | Parkhouse et al. (2014) |
| Identifier of the publication | pubmed:24960071 imex:IM-22927 |
| NCBI Taxonomy identifier for interactor A | taxid:10090(mouse) taxid:10090(Mus musculus) |
| NCBI Taxonomy identifier for interactor B | taxid:9606(human) taxid:9606(Homo sapiens) |
| Interaction types | psi-mi:"MI:0915"(physical association) |
| Source databases and identifiers | psi-mi:"MI:0471"(MINT) |
| Interaction identifier(s) in the corresponding source database | intact:EBI-9549096 imex:IM-22927-1 |
| Confidence score | intact-miscore:0.40 |
| Complex expansion | - |
| Biological role A | Unspecified role |
| Biological role B | Unspecified role |
| Experimental role A | Prey |
| Experimental role B | Bait |
| Interactor type A | Protein |
| Interactor type B | Protein |
| Annotations for the interaction | figure legend:f2 f3 comment:"\"CARD9 was immunoprecipitated by full-length, CARD-NACHT, and NACHT-LRR NOD2 constructs (Figure 2A). Neither the CARDs nor the LRRs alone interacted with CARD9, suggesting that the NACHT domain of NOD2 has a critical role in mediating interaction with CARD9. To further investigate the importance of the NOD2 NACHT domain we tested the impact of a panel of Crohn’s Disease associated single nucleotide polymorphisms (SNP) [33–36] spanning the NACHT on the interaction with CARD9. This included the widely studied R702W SNP. We also tested the hyperactive Blau Syndrome associated SNP R334W [37]. All of the polymorphisms still interacted with CARD9 indicating that these residues, and potentially the surrounding regions of the NACHT,were not crucial for CARD9 interaction (Figure 2B & 2C).\"" comment:"\"we generated further C-terminal NOD2 truncations (Figure 3A) and tested their interaction with CARD9. All of these truncations, including A274stop which lacks any of the NACHT, retained the ability to interact with CARD9 (Figure 3B). Together with the failure of the CARD only construct (residues 1-227 ; Figure 2A & 3B) to interact with CARD9 this indicated that residues 228-274 contain a critical region for CARD9 interaction.\"" comment:"\"In summary the NOD2 CARD-NACHT linker (residues 228-274) and the NACHT domain itself contain regions capable of interaction with CARD9\"" curation depth:imex curation full coverage:Only protein-protein interactions |
| NCBI Taxonomy identifier for the host organism | taxid:9606(human-293) taxid:9606(Homo sapiens HEK293 embryonic kidney cell) |
| Parameters of the interaction | - |
| Creation date | 2014/06/12 |
| Update date | 2024/11/30 |
| negative Boolean value | false |
| Feature(s) for interactor A | v5 tag:?-? |
| Feature(s) for interactor B | flag tag:?-? binding-associated region:228-274 |
| Stoichiometry for interactor A | - |
| Stoichiometry for interactor B | - |
| Participant identification method for interactor A | Western blot |
| Participant identification method for interactor B | Western blot |