Curation Details
Interaction ID: IM-22599-1

Unique identifier for interactor Auniprotkb:P62340
Unique identifier for interactor Buniprotkb:P52655
Alternative identifier for interactor Aintact:EBI-7640256
uniprotkb:O95753
intact:MINT-1348231
uniprotkb:Q9Z2Z0
ensembl:ENSMUSP00000093470.4
ensembl:ENSMUSP00000114223.2
Alternative identifier for interactor Bintact:EBI-389518
uniprotkb:Q3KNQ9
ensembl:ENSP00000452454.1
Aliases for Apsi-mi:tbpl1_mouse(display_long)
uniprotkb:Tbpl1(gene name)
psi-mi:Tbpl1(display_short)
uniprotkb:Tlp(gene name synonym)
uniprotkb:Tlp21(gene name synonym)
uniprotkb:Tlf(gene name synonym)
uniprotkb:Trf2(gene name synonym)
uniprotkb:Trp(gene name synonym)
uniprotkb:TATA box-binding protein-related factor 2(gene name synonym)
uniprotkb:TBP-like factor(gene name synonym)
uniprotkb:TBP-related protein(gene name synonym)
uniprotkb:21 kDa TBP-like protein(gene name synonym)
Aliases for Bpsi-mi:tf2aa_human(display_long)
uniprotkb:GTF2A1(gene name)
psi-mi:GTF2A1(display_short)
uniprotkb:TF2A1(gene name synonym)
uniprotkb:General transcription factor IIA subunit 1(gene name synonym)
uniprotkb:Transcription initiation factor TFIIA 42 kDa subunit(gene name synonym)
uniprotkb:TFIIAL(gene name synonym)
Interaction detection methodspsi-mi:"MI:0007"(anti tag coimmunoprecipitation)
First authorSuzuki et al. (2014)
Identifier of the publicationpubmed:24835508
imex:IM-22599
NCBI Taxonomy identifier for interactor Ataxid:10090(mouse)
taxid:10090(Mus musculus)
NCBI Taxonomy identifier for interactor Btaxid:9606(human)
taxid:9606(Homo sapiens)
Interaction typespsi-mi:"MI:0914"(association)
Source databases and identifierspsi-mi:"MI:0471"(MINT)
Interaction identifier(s) in the corresponding source databaseintact:EBI-9524851
imex:IM-22599-1
Confidence scoreintact-miscore:0.35
Complex expansionpsi-mi:"MI:1060"(spoke expansion)
Biological role AUnspecified role
Biological role BUnspecified role
Experimental role ABait
Experimental role BPrey
Interactor type AProtein
Interactor type BProtein
Annotations for the interactionfigure legend:f1
comment:"\"In this study, we first investigated in detail the intracellular TFIIA-binding strength of those mutants by co-immunoprecipitation assays. It was confirmed that wild-type TLP and R55E exhibited significant binding to TFIIA, whereas N37E did not bind to TFIIA (Fig. 1). We further investigated processed and unprocessed forms of TFIIAα/β and found that R52E binds to the α and β subunits of TFIIA but does not bind to uncleaved TFIIAαβ (Fig. 1, lane 12).\""
curation depth:imex curation
full coverage:Only protein-protein interactions
NCBI Taxonomy identifier for the host organismtaxid:9606(human-hct116)
taxid:9606(Homo sapiens HCT116 colon carcinoma cell)
Parameters of the interaction-
Creation date2014/05/13
Update date2024/11/30
negative Boolean valuefalse
Feature(s) for interactor Atandem tag:?-?
mutation disrupting interaction:37-37
Feature(s) for interactor Bbinding-associated region:275-376
Stoichiometry for interactor A-
Stoichiometry for interactor B-
Participant identification method for interactor AWestern blot
Participant identification method for interactor BWestern blot