Curation Details
Interaction ID: IM-22570-2

Unique identifier for interactor Auniprotkb:Q13153
Unique identifier for interactor Buniprotkb:P63000
Alternative identifier for interactor Aintact:EBI-1307
ensembl:ENSP00000348696.4
intact:EBI-28976107
uniprotkb:O75561
uniprotkb:Q13567
uniprotkb:Q32M53
uniprotkb:Q32M54
uniprotkb:Q86W79
Alternative identifier for interactor Bintact:EBI-413628
ensembl:ENSP00000258737.7
uniprotkb:O95501
uniprotkb:P15154
uniprotkb:Q3Y4D3
uniprotkb:Q5JAA8
uniprotkb:Q9BTB4
Aliases for Apsi-mi:pak1_human(display_long)
uniprotkb:PAK1(gene name)
psi-mi:PAK1(display_short)
uniprotkb:p21-activated kinase 1(gene name synonym)
uniprotkb:p65-PAK(gene name synonym)
uniprotkb:Alpha-PAK(gene name synonym)
Aliases for Bpsi-mi:rac1_human(display_long)
uniprotkb:RAC1(gene name)
psi-mi:RAC1(display_short)
uniprotkb:MIG5(orf name)
uniprotkb:p21-Rac1(gene name synonym)
uniprotkb:Ras-like protein TC25(gene name synonym)
uniprotkb:Cell migration-inducing gene 5 protein(gene name synonym)
uniprotkb:TC25(gene name synonym)
Interaction detection methodspsi-mi:"MI:0096"(pull down)
First authorKaito et al. (2014)
Identifier of the publicationimex:IM-22570
pubmed:24792722
NCBI Taxonomy identifier for interactor Ataxid:9606(human)
taxid:9606(Homo sapiens)
NCBI Taxonomy identifier for interactor Btaxid:9606(human)
taxid:9606(Homo sapiens)
Interaction typespsi-mi:"MI:0915"(physical association)
Source databases and identifierspsi-mi:"MI:0471"(MINT)
Interaction identifier(s) in the corresponding source databaseintact:EBI-9396362
imex:IM-22570-2
Confidence scoreintact-miscore:0.98
Complex expansion-
Biological role AUnspecified role
Biological role BUnspecified role
Experimental role ABait
Experimental role BPrey
Interactor type AProtein
Interactor type BProtein
Annotations for the interactionfigure legend:f6
comment:"\"We estimated Rac-GTP level in the lysates from transfected cells using PAK-PBD pull-down assay (Fig. 6). Imaging analysis of the bands showed that Pix(S340E) expression increased Rac-GTP level by 66%, and the ratio to the total Rac amount was calculated to be 2.3-fold of that with the control.\""
comment:"\"Endogenous Rac-GTP level was estimated by PAK-PBD pull-down assay using a Rac1 Activation Assay Biochem Kit (Cytoskeleton, Denver, CO) according to the manufacturer’s protocol\""
full coverage:Only protein-protein interactions
curation depth:imex curation
NCBI Taxonomy identifier for the host organismtaxid:-1(in vitro)
taxid:-1(In vitro)
Parameters of the interaction-
Creation date2014/04/16
Update date2014/10/16
negative Boolean valuefalse
Feature(s) for interactor Aglutathione s tranferase tag:?-?
binding-associated region:67-150
Feature(s) for interactor B-
Stoichiometry for interactor A-
Stoichiometry for interactor B-
Participant identification method for interactor APredetermined participant
Participant identification method for interactor BWestern blot