| Unique identifier for interactor A | uniprotkb:P63000 |
| Unique identifier for interactor B | uniprotkb:Q14155 |
| Alternative identifier for interactor A | intact:EBI-413628 uniprotkb:O95501 uniprotkb:P15154 uniprotkb:Q3Y4D3 uniprotkb:Q9BTB4 uniprotkb:Q5JAA8 ensembl:ENSP00000258737.7 |
| Alternative identifier for interactor B | intact:EBI-717515 uniprotkb:B1ALK6 uniprotkb:B1ALK8 uniprotkb:Q3LIA4 uniprotkb:Q5W9H0 uniprotkb:Q6P9G3 uniprotkb:Q6PII2 uniprotkb:Q86W63 uniprotkb:Q8N3M1 intact:EBI-25643109 ensembl:ENSP00000364893.2 |
| Aliases for A | psi-mi:rac1_human(display_long) uniprotkb:RAC1(gene name) psi-mi:RAC1(display_short) uniprotkb:MIG5(orf name) uniprotkb:p21-Rac1(gene name synonym) uniprotkb:Ras-like protein TC25(gene name synonym) uniprotkb:Cell migration-inducing gene 5 protein(gene name synonym) uniprotkb:TC25(gene name synonym) |
| Aliases for B | psi-mi:arhg7_human(display_long) uniprotkb:PAK-interacting exchange factor beta(gene name synonym) uniprotkb:Beta-Pix(gene name synonym) uniprotkb:COOL-1(gene name synonym) uniprotkb:p85(gene name synonym) uniprotkb:Nbla10314(orf name) uniprotkb:ARHGEF7(gene name) psi-mi:ARHGEF7(display_short) uniprotkb:COOL1(gene name synonym) uniprotkb:KIAA0142(gene name synonym) uniprotkb:P85SPR(gene name synonym) uniprotkb:PAK3BP(gene name synonym) uniprotkb:PIXB(gene name synonym) |
| Interaction detection methods | psi-mi:"MI:0096"(pull down) |
| First author | Kaito et al. (2014) |
| Identifier of the publication | imex:IM-22570 pubmed:24792722 |
| NCBI Taxonomy identifier for interactor A | taxid:9606(human) taxid:9606(Homo sapiens) |
| NCBI Taxonomy identifier for interactor B | taxid:9606(human) taxid:9606(Homo sapiens) |
| Interaction types | psi-mi:"MI:0407"(direct interaction) |
| Source databases and identifiers | psi-mi:"MI:0471"(MINT) |
| Interaction identifier(s) in the corresponding source database | intact:EBI-9396343 imex:IM-22570-1 |
| Confidence score | intact-miscore:0.68 |
| Complex expansion | - |
| Biological role A | Unspecified role |
| Biological role B | Unspecified role |
| Experimental role A | Bait |
| Experimental role B | Prey |
| Interactor type A | Protein |
| Interactor type B | Protein |
| Annotations for the interaction | figure legend:f4b f4c comment:"\"As Pix(S340E) showed higher O2 - production, we examined whether the Rac binding affinity was enhanced by the mutation. Pix and its mutants recombinantly expressed and purified (Fig. 4A) were used in pull-down assays with GST-Rac (GDP-bound) or GSTRacQ61L (GTP-bound) (Fig. 4B). The assays showed that Pix interacted with GST-Rac and less tightly with GST-RacQ61L (Fig. 4B, 4C). Furthermore, the S340E mutant showed a higher Rac binding affinity (2.3-fold with GDP-form), while S340A mutant had a similar affinity to wild-type Pix.\"" curation depth:imex curation full coverage:Only protein-protein interactions |
| NCBI Taxonomy identifier for the host organism | taxid:-1(in vitro) taxid:-1(In vitro) |
| Parameters of the interaction | - |
| Creation date | 2014/04/16 |
| Update date | 2024/11/30 |
| negative Boolean value | false |
| Feature(s) for interactor A | glutathione s tranferase tag:?-? mutation decreasing interaction rate:61-61 |
| Feature(s) for interactor B | mutation increasing interaction:340-340 |
| Stoichiometry for interactor A | - |
| Stoichiometry for interactor B | - |
| Participant identification method for interactor A | Molecular weight estimation by coomasie staining |
| Participant identification method for interactor B | Molecular weight estimation by coomasie staining |