Curation Details
Interaction ID: IM-22547-1

Unique identifier for interactor Auniprotkb:Q9UHW9
Unique identifier for interactor Buniprotkb:Q9UEW8
Alternative identifier for interactor Aintact:EBI-2823282
uniprotkb:Q9UFR2
uniprotkb:Q8TDD4
uniprotkb:Q9Y665
uniprotkb:Q9Y642
uniprotkb:A0AV76
ensembl:ENSP00000346112.3
ensembl:ENSP00000453473.1
ensembl:ENSP00000454168.1
uniprotkb:Q2VI00
uniprotkb:Q7Z2E7
uniprotkb:Q7Z4G5
Alternative identifier for interactor Bintact:EBI-2680974
uniprotkb:Q9UER4
uniprotkb:O14774
uniprotkb:Q53S90
uniprotkb:Q53SL7
uniprotkb:Q53SS1
ensembl:ENSP00000348278.4
uniprotkb:X5D9C8
Aliases for Apsi-mi:s12a6_human(display_long)
uniprotkb:SLC12A6(gene name)
psi-mi:SLC12A6(display_short)
uniprotkb:KCC3(gene name synonym)
uniprotkb:Electroneutral potassium-chloride cotransporter 3(gene name synonym)
uniprotkb:K-Cl cotransporter 3(gene name synonym)
Aliases for Bpsi-mi:stk39_human(display_long)
uniprotkb:SPAK(gene name synonym)
uniprotkb:DCHT(gene name synonym)
uniprotkb:Serine/threonine-protein kinase 39(gene name synonym)
uniprotkb:PASK(gene name synonym)
uniprotkb:STK39(gene name)
psi-mi:STK39(display_short)
Interaction detection methodspsi-mi:"MI:0006"(anti bait coimmunoprecipitation)
First authorChiu et al. (2014)
Identifier of the publicationpubmed:24655550
imex:IM-22547
NCBI Taxonomy identifier for interactor Ataxid:9606(human)
taxid:9606(Homo sapiens)
NCBI Taxonomy identifier for interactor Btaxid:9606(human)
taxid:9606(Homo sapiens)
Interaction typespsi-mi:"MI:0915"(physical association)
Source databases and identifierspsi-mi:"MI:0471"(MINT)
Interaction identifier(s) in the corresponding source databaseintact:EBI-9254964
imex:IM-22547-1
Confidence scoreintact-miscore:0.40
Complex expansion-
Biological role AUnspecified role
Biological role BUnspecified role
Experimental role ABait
Experimental role BPrey
Interactor type AProtein
Interactor type BProtein
Annotations for the interactionfigure legend:f2b
comment:"\"To establish an IPTG-inducible KCC3 cell line, HEK 293 cells were selected to be co-transfected with pTet-on-Lac Hyg and pLacM hKCC3 using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocol, and stable transfectants were selected with Hygomycin B (Invitrogen). This cell line was named iKCC3 HEK293\""
full coverage:Only protein-protein interactions
curation depth:imex curation
NCBI Taxonomy identifier for the host organismtaxid:9606(human-293t)
taxid:9606(Homo sapiens HEK293T embryonic kidney cell)
Parameters of the interaction-
Creation date2014/03/10
Update date2014/10/16
negative Boolean valuefalse
Feature(s) for interactor A-
Feature(s) for interactor B-
Stoichiometry for interactor A-
Stoichiometry for interactor B-
Participant identification method for interactor AWestern blot
Participant identification method for interactor BWestern blot