| Unique identifier for interactor A | uniprotkb:P56817 |
| Unique identifier for interactor B | uniprotkb:O75955 |
| Alternative identifier for interactor A | intact:EBI-2433139 uniprotkb:B0YIU9 uniprotkb:Q9BYB9 uniprotkb:Q9BYC0 uniprotkb:Q9BYC1 uniprotkb:Q9UJT5 uniprotkb:Q9ULS1 uniprotkb:E9PE65 uniprotkb:H7BXJ9 uniprotkb:A0M8W7 ensembl:ENSP00000318585.6 |
| Alternative identifier for interactor B | intact:EBI-603643 uniprotkb:Q969J8 uniprotkb:Q9UHW1 uniprotkb:Q9UNV8 ensembl:ENSP00000365569.3 ensembl:ENSP00000372873.4 ensembl:ENSP00000373056.4 ensembl:ENSP00000388861.2 ensembl:ENSP00000391438.2 uniprotkb:B4DVY7 |
| Aliases for A | psi-mi:bace1_human(display_long) uniprotkb:BACE(gene name synonym) uniprotkb:KIAA1149(gene name synonym) uniprotkb:Beta-site amyloid precursor protein cleaving enzyme 1(gene name synonym) uniprotkb:Membrane-associated aspartic protease 2(gene name synonym) uniprotkb:Memapsin-2(gene name synonym) uniprotkb:Aspartyl protease 2(gene name synonym) uniprotkb:BACE1(gene name) psi-mi:BACE1(display_short) |
| Aliases for B | psi-mi:flot1_human(display_long) uniprotkb:FLOT1(gene name) psi-mi:FLOT1(display_short) |
| Interaction detection methods | psi-mi:"MI:0813"(proximity ligation assay) |
| First author | John et al. (2014) |
| Identifier of the publication | pubmed:24612608 imex:IM-22281 |
| NCBI Taxonomy identifier for interactor A | taxid:9606(human) taxid:9606(Homo sapiens) |
| NCBI Taxonomy identifier for interactor B | taxid:9606(human) taxid:9606(Homo sapiens) |
| Interaction types | psi-mi:"MI:2364"(proximity) |
| Source databases and identifiers | psi-mi:"MI:0471"(MINT) |
| Interaction identifier(s) in the corresponding source database | intact:EBI-9213821 imex:IM-22281-4 |
| Confidence score | intact-miscore:0.27 |
| Complex expansion | - |
| Biological role A | Unspecified role |
| Biological role B | Unspecified role |
| Experimental role A | Neutral component |
| Experimental role B | Neutral component |
| Interactor type A | Protein |
| Interactor type B | Protein |
| Annotations for the interaction | figure legend:f2b comment:"\"To dissect the role of flotillins in BACE1 binding in more detail and to test if the interaction is a direct one, we performed direct pulldown assays using bacterially expressed, purified proteins (Figure 1D). Purified flotillin-1 again strongly bound to the WT BACE1-GST tail, whereas no binding was observed with the LLAA tail. Only a very weak binding that was close to the GST background level was observed with purified flotillin-2. Taken together, these data show that flotillin-1 directly interacts with the cytoplasmic tail of BACE1 and that this interaction is mediated by the well known dileucine sorting motif in BACE1. To corroborate these findings in an in vivo context, we performed coimmunoprecipitation experiments with endogenous flotillins and BACE1 (Figure 2A). For this, we used polyclonal flotillin-2 antibodies since they result in a quantitative precipitation of the pool of flotillin-1/flotillin-2 complexes in the cells. BACE1 was found to be immunoprecipitated with endogenous flotillin-2, verifying the relevance of the pulldown data. Since the signals observed in the coprecipitation experiment were very weak, we used the Proximity Ligation Assay (PLA) to verify the interaction of endogenous BACE1 and flotillins. PLA is based on the use of specific antibodies and can detect two proteins that are at very close vicinity (less than 40 nm) in cells and is generally used to study interaction of proteins [38-40]. Interacting proteins are visualized as fluorescent signals whose localization in the cells can also be assessed, giving information on the compartment in which the interaction takes place. However, the signal intensities as such do not correlate with the strength or quantity of the interaction. Quantification of the signals is done by counting the amount of fluorescent signals per cells and comparing these to negative controls (performed without the first antibody). PLA analysis of endogenous flotillin-1 or flotillin-2 and BACE1 showed that the fluorescent dots that arise upon interaction of these proteins were mainly detected in the perinuclear region of the cells, with few dots residing in the cell periphery (Fig. 2B).\"" dataset:Alzheimers - Interactions investigated in the context of Alzheimers disease curation depth:imex curation full coverage:Only protein-protein interactions |
| NCBI Taxonomy identifier for the host organism | taxid:9606(human-hela) taxid:9606(Homo sapiens HeLa epitheloid cervical carcinoma cell) |
| Parameters of the interaction | - |
| Creation date | 2014/02/21 |
| Update date | 2024/09/12 |
| negative Boolean value | false |
| Feature(s) for interactor A | - |
| Feature(s) for interactor B | - |
| Stoichiometry for interactor A | - |
| Stoichiometry for interactor B | - |
| Participant identification method for interactor A | Immunostaining |
| Participant identification method for interactor B | Immunostaining |