| Unique identifier for interactor A | uniprotkb:P56817 |
| Unique identifier for interactor B | uniprotkb:Q9Z1E1 |
| Alternative identifier for interactor A | intact:EBI-2433139 uniprotkb:B0YIU9 uniprotkb:Q9BYB9 uniprotkb:Q9BYC0 uniprotkb:Q9BYC1 uniprotkb:Q9UJT5 uniprotkb:Q9ULS1 uniprotkb:E9PE65 uniprotkb:H7BXJ9 uniprotkb:A0M8W7 ensembl:ENSP00000318585.6 |
| Alternative identifier for interactor B | intact:EBI-920268 uniprotkb:Q9Z1E2 ensembl:ENSRNOP00000071275.1 |
| Aliases for A | psi-mi:bace1_human(display_long) uniprotkb:BACE(gene name synonym) uniprotkb:KIAA1149(gene name synonym) uniprotkb:Beta-site amyloid precursor protein cleaving enzyme 1(gene name synonym) uniprotkb:Membrane-associated aspartic protease 2(gene name synonym) uniprotkb:Memapsin-2(gene name synonym) uniprotkb:Aspartyl protease 2(gene name synonym) uniprotkb:BACE1(gene name) psi-mi:BACE1(display_short) |
| Aliases for B | psi-mi:flot1_rat(display_long) uniprotkb:Flot1(gene name) psi-mi:Flot1(display_short) uniprotkb:Reg2(gene name synonym) uniprotkb:Reggie-2(gene name synonym) |
| Interaction detection methods | psi-mi:"MI:0096"(pull down) |
| First author | John et al. (2014) |
| Identifier of the publication | pubmed:24612608 imex:IM-22281 |
| NCBI Taxonomy identifier for interactor A | taxid:9606(human) taxid:9606(Homo sapiens) |
| NCBI Taxonomy identifier for interactor B | taxid:10116(rat) taxid:10116("Rattus norvegicus (Rat)") |
| Interaction types | psi-mi:"MI:0407"(direct interaction) |
| Source databases and identifiers | psi-mi:"MI:0471"(MINT) |
| Interaction identifier(s) in the corresponding source database | intact:EBI-9213775 imex:IM-22281-1 |
| Confidence score | intact-miscore:0.44 |
| Complex expansion | - |
| Biological role A | Unspecified role |
| Biological role B | Unspecified role |
| Experimental role A | Bait |
| Experimental role B | Prey |
| Interactor type A | Protein |
| Interactor type B | Protein |
| Annotations for the interaction | figure legend:f1d comment:"\"To dissect the role of flotillins in BACE1 binding in more detail and to test if the interaction is a direct one, we performed direct pulldown assays using bacterially expressed, purified proteins (Figure 1D). Purified flotillin-1 again strongly bound to the WT BACE1-GST tail, whereas no binding was observed with the LLAA tail. Only a very weak binding that was close to the GST background level was observed with purified flotillin-2. Taken together, these data show that flotillin-1 directly interacts with the cytoplasmic tail of BACE1 and that this interaction is mediated by the well known dileucine sorting motif in BACE1\"" dataset:Alzheimers - Interactions investigated in the context of Alzheimers disease curation depth:imex curation full coverage:Only protein-protein interactions |
| NCBI Taxonomy identifier for the host organism | taxid:-1(in vitro) taxid:-1(In vitro) |
| Parameters of the interaction | - |
| Creation date | 2014/02/21 |
| Update date | 2025/03/31 |
| negative Boolean value | false |
| Feature(s) for interactor A | glutathione s tranferase tag:?-? binding-associated region:479-501 mutation disrupting interaction:499-500 |
| Feature(s) for interactor B | - |
| Stoichiometry for interactor A | - |
| Stoichiometry for interactor B | - |
| Participant identification method for interactor A | Predetermined participant |
| Participant identification method for interactor B | Western blot |