Curation Details
Interaction ID: IM-22232-2

Unique identifier for interactor Auniprotkb:O15205
Unique identifier for interactor Buniprotkb:Q9H832
Alternative identifier for interactor Aintact:EBI-6657186
uniprotkb:Q96EC7
uniprotkb:Q5STL2
uniprotkb:B0UZT6
uniprotkb:Q5SUK2
ensembl:ENSP00000366249.4
ensembl:ENSP00000373039.3
ensembl:ENSP00000396152.2
ensembl:ENSP00000410416.2
Alternative identifier for interactor Bintact:EBI-720977
uniprotkb:A6N8M6
uniprotkb:Q7L354
uniprotkb:Q8TCM4
uniprotkb:Q9H893
uniprotkb:A6NC60
ensembl:ENSP00000354201.5
Aliases for Apsi-mi:ubd_human(display_long)
uniprotkb:UBD(gene name)
psi-mi:UBD(display_short)
uniprotkb:Diubiquitin(gene name synonym)
uniprotkb:FAT10(gene name synonym)
uniprotkb:Ubiquitin-like protein FAT10(gene name synonym)
Aliases for Bpsi-mi:ube2z_human(display_long)
uniprotkb:Ubiquitin-protein ligase Z(gene name synonym)
uniprotkb:Ubiquitin carrier protein Z(gene name synonym)
uniprotkb:Uba6-specific E2 conjugating enzyme 1(gene name synonym)
uniprotkb:UBE2Z(gene name)
psi-mi:UBE2Z(display_short)
uniprotkb:HOYS7(orf name)
uniprotkb:E2 ubiquitin-conjugating enzyme Z(gene name synonym)
Interaction detection methodspsi-mi:"MI:0007"(anti tag coimmunoprecipitation)
First authorAichem et al. (2014)
Identifier of the publicationimex:IM-22232
pubmed:24528925
NCBI Taxonomy identifier for interactor Ataxid:9606(human)
taxid:9606(Homo sapiens)
NCBI Taxonomy identifier for interactor Btaxid:9606(human)
taxid:9606(Homo sapiens)
Interaction typespsi-mi:"MI:0915"(physical association)
Source databases and identifierspsi-mi:"MI:0471"(MINT)
Interaction identifier(s) in the corresponding source databaseintact:EBI-9119842
imex:IM-22232-2
Confidence scoreintact-miscore:0.52
Complex expansion-
Biological role AUnspecified role
Biological role BUnspecified role
Experimental role APrey
Experimental role BBait
Interactor type AProtein
Interactor type BProtein
Annotations for the interactionfigure legend:f3
comment:"\"As shown in Fig. 3B, each mutant was still able to form a stable conjugate with FAT10. To compare the amounts of the mutant USE1-FAT10 conjugates, the ECL signals of the USE1-FAT10 conjugates were quantified and normalized to the respective USE1 variants in the lysate (load) (Fig. 3B, C). A statistical analysis revealed significantly lower amounts of USE1-K323R-FAT10 (p-value 0.0156) while all other mutant USE1-FAT10 conjugates did not significantly differ in quantity from wildtype USE1-FAT10\""
curation depth:imex curation
full coverage:Only protein-protein interactions
NCBI Taxonomy identifier for the host organismtaxid:9606(human-293)
taxid:9606(Homo sapiens HEK293 embryonic kidney cell)
Parameters of the interaction-
Creation date2014/02/06
Update date2024/11/30
negative Boolean valuefalse
Feature(s) for interactor Atandem tag:?-?
Feature(s) for interactor Bmutation decreasing interaction:323-323
ha tag:?-?
Stoichiometry for interactor A-
Stoichiometry for interactor B-
Participant identification method for interactor AAnti tag western blot
Participant identification method for interactor BAnti tag western blot