| Unique identifier for interactor A | uniprotkb:P00750 |
| Unique identifier for interactor B | uniprotkb:P00750 |
| Alternative identifier for interactor A | intact:EBI-5605520 ensembl:ENSP00000220809.4 ensembl:ENSP00000392045.2 ensembl:ENSP00000504311.1 uniprotkb:A8K022 uniprotkb:B2R8E8 uniprotkb:Q15103 uniprotkb:Q503B0 uniprotkb:Q6PJA5 uniprotkb:Q7Z7N2 uniprotkb:Q86YK8 uniprotkb:Q9BU99 uniprotkb:Q9BZW1 |
| Alternative identifier for interactor B | intact:EBI-5605520 ensembl:ENSP00000220809.4 ensembl:ENSP00000392045.2 ensembl:ENSP00000504311.1 uniprotkb:A8K022 uniprotkb:B2R8E8 uniprotkb:Q15103 uniprotkb:Q503B0 uniprotkb:Q6PJA5 uniprotkb:Q7Z7N2 uniprotkb:Q86YK8 uniprotkb:Q9BU99 uniprotkb:Q9BZW1 |
| Aliases for A | psi-mi:tpa_human(display_long) uniprotkb:PLAT(gene name) psi-mi:PLAT(display_short) |
| Aliases for B | psi-mi:tpa_human(display_long) uniprotkb:PLAT(gene name) psi-mi:PLAT(display_short) |
| Interaction detection methods | psi-mi:"MI:0038"(dynamic light scattering) |
| First author | Tischer et al. (2014) |
| Identifier of the publication | imex:IM-22212 pubmed:24506586 |
| NCBI Taxonomy identifier for interactor A | taxid:9606(human) taxid:9606(Homo sapiens) |
| NCBI Taxonomy identifier for interactor B | taxid:9606(human) taxid:9606(Homo sapiens) |
| Interaction types | psi-mi:"MI:0407"(direct interaction) |
| Source databases and identifiers | psi-mi:"MI:0471"(MINT) |
| Interaction identifier(s) in the corresponding source database | intact:EBI-9107376 imex:IM-22212-1 |
| Confidence score | intact-miscore:0.44 |
| Complex expansion | - |
| Biological role A | Unspecified role |
| Biological role B | Unspecified role |
| Experimental role A | Neutral component |
| Experimental role B | Neutral component |
| Interactor type A | Protein |
| Interactor type B | Protein |
| Annotations for the interaction | figure legend:f5 f2b comment:"\"The solubility maximum of CM-rPA in 2 M EMIMCl was also measured as a function of incubation time (Fig. 2A; inset). The solubility of CM-rPA decreased over several days and after 67 days of incubation, 1.3 ± 0.1 mg mL-1 CM-rPA was detected in the soluble fraction. In addition to the solubility measurements, the state of CM-rPA in solution was analyzed by dynamic light scattering measurements (Fig. 2B). From these measurements a z-average value of 16.9 ± 0.5 nm was calculated for a sample which was incubated 19 h in 2 M EMIMCl at 4 °C. As the determined z-average values are a measure for the intensity weighed hydrodynamic diameter of particles, they indicate that CM-rPA exists as an ensemble of small aggregated protein species in solution. After an incubation of 67 days, an increase of the z-average value to 18.3 ± 0.1 was observed, indicating a shift of the aggregates to larger sizes.\"" comment:"\"To test this hypothesis, we used dynamic light scattering to determine the particle size distributions (PSDs) for 54 mg mL-1 tc-rPA in 5.4 M EMIMCl as a function of time (Fig. 5). Since it was not possible to determine the exact viscosity of the EMIMCl-solution containing a certain amount of tc-rPA, the first z-average was adjusted to a diameter of 5.7 nm, which corresponded to globular, monomeric tc-rPA with a size 39.6 kDa. The measurement of the intensity weighed PSD showed a shift towards higher sizes over time. This was also reflected by the z-average diameters which increased from 5.7 nm to approximately 75 nm within 214 h. Under the assumption of a globular shape for the 75 nm particles such a z-average correlates to a hydrodynamic diameter of approximately 12 MDa indicating that tc-rPA formed soluble aggregates after unfolding in 5.4 M EMIMCl.\"" full coverage:Only protein-protein interactions curation depth:imex curation |
| NCBI Taxonomy identifier for the host organism | taxid:-1(in vitro) taxid:-1(In vitro) |
| Parameters of the interaction | - |
| Creation date | 2014/02/03 |
| Update date | 2014/10/16 |
| negative Boolean value | false |
| Feature(s) for interactor A | - |
| Feature(s) for interactor B | - |
| Stoichiometry for interactor A | - |
| Stoichiometry for interactor B | - |
| Participant identification method for interactor A | Predetermined participant |
| Participant identification method for interactor B | Predetermined participant |