| Unique identifier for interactor A | uniprotkb:Q9Y4X5 |
| Unique identifier for interactor B | uniprotkb:P68036 |
| Alternative identifier for interactor A | intact:EBI-2514233 uniprotkb:Q9UEN0 uniprotkb:Q9H3T6 uniprotkb:O76026 uniprotkb:Q9UP39 uniprotkb:B2R6U3 ensembl:ENSP00000369217.4 |
| Alternative identifier for interactor B | intact:EBI-711173 uniprotkb:P51966 uniprotkb:P70653 uniprotkb:Q9HAV1 uniprotkb:B2R4A7 uniprotkb:B4DDG1 uniprotkb:B4DSZ4 uniprotkb:E7EWS7 ensembl:ENSP00000344259.5 intact:EBI-21353523 |
| Aliases for A | psi-mi:ari1_human(display_long) uniprotkb:Protein ariadne-1 homolog(gene name synonym) uniprotkb:ARIH1(gene name) psi-mi:ARIH1(display_short) uniprotkb:ARI(gene name synonym) uniprotkb:MOP6(gene name synonym) uniprotkb:UBCH7BP(gene name synonym) uniprotkb:Ubiquitin-conjugating enzyme E2-binding protein 1(gene name synonym) uniprotkb:UbcH7-binding protein(gene name synonym) uniprotkb:UbcM4-interacting protein(gene name synonym) uniprotkb:HHARI(gene name synonym) uniprotkb:H7-AP2(gene name synonym) uniprotkb:Monocyte protein 6(gene name synonym) uniprotkb:HUSSY-27(orf name) |
| Aliases for B | psi-mi:ub2l3_human(display_long) uniprotkb:Ubiquitin-protein ligase L3(gene name synonym) uniprotkb:Ubiquitin carrier protein L3(gene name synonym) uniprotkb:UbcH7(gene name synonym) uniprotkb:Ubiquitin-conjugating enzyme E2-F1(gene name synonym) uniprotkb:L-UBC(gene name synonym) uniprotkb:UBE2L3(gene name) psi-mi:UBE2L3(display_short) uniprotkb:UBCE7(gene name synonym) uniprotkb:UBCH7(gene name synonym) uniprotkb:E2 ubiquitin-conjugating enzyme L3(gene name synonym) |
| Interaction detection methods | psi-mi:"MI:0007"(anti tag coimmunoprecipitation) |
| First author | Kelsall et al. (2013) |
| Identifier of the publication | imex:IM-22207 pubmed:24076655 |
| NCBI Taxonomy identifier for interactor A | taxid:9606(human) taxid:9606(Homo sapiens) |
| NCBI Taxonomy identifier for interactor B | taxid:9606(human) taxid:9606(Homo sapiens) |
| Interaction types | psi-mi:"MI:0914"(association) |
| Source databases and identifiers | psi-mi:"MI:0471"(MINT) |
| Interaction identifier(s) in the corresponding source database | intact:EBI-9118093 imex:IM-22207-16 |
| Confidence score | intact-miscore:0.64 |
| Complex expansion | psi-mi:"MI:1060"(spoke expansion) |
| Biological role A | Unspecified role |
| Biological role B | Unspecified role |
| Experimental role A | Bait |
| Experimental role B | Prey |
| Interactor type A | Protein |
| Interactor type B | Protein |
| Annotations for the interaction | figure legend:sf5f comment:"\"We next tested the effects of mutations in the HHARI Ariadne domain, which were recently shown to relieve auto‐inhibition and result in enhanced HHARI auto‐ubiquitylation in vitro (Duda et al, 2013) (Figure 7H). We observed less GFP‐HHARI overall, potentially reflecting effects of auto‐ubiquitylation, and a concomitant increase in CUL1‐NEDD8 levels. Inactivation of E3 ligase activity by means of an additional catalytic C357S mutation restored accumulation of these Ariadne domain variants, as did treatment with the proteasomal inhibitor MG132 (Supplementary Figure S5F).\"" curation depth:imex curation full coverage:Only protein-protein interactions |
| NCBI Taxonomy identifier for the host organism | taxid:9606(human-293) taxid:9606(Homo sapiens HEK293 embryonic kidney cell) |
| Parameters of the interaction | - |
| Creation date | 2014/02/04 |
| Update date | 2024/11/30 |
| negative Boolean value | false |
| Feature(s) for interactor A | mutation disrupting interaction:430-430,431-431,503-503 green fluorescent protein tag:?-? |
| Feature(s) for interactor B | - |
| Stoichiometry for interactor A | - |
| Stoichiometry for interactor B | - |
| Participant identification method for interactor A | Anti tag western blot |
| Participant identification method for interactor B | Western blot |