| Unique identifier for interactor A | uniprotkb:Q13616 |
| Unique identifier for interactor B | uniprotkb:Q9Y4X5 |
| Alternative identifier for interactor A | intact:EBI-359390 uniprotkb:O60719 uniprotkb:Q08AL6 uniprotkb:Q8IYW1 uniprotkb:D3DWG3 ensembl:ENSP00000326804.3 ensembl:ENSP00000387160.1 ensembl:ENSP00000473318.1 ensembl:ENSP00000499255.1 ensembl:ENSP00000499277.1 ensembl:ENSP00000499398.1 |
| Alternative identifier for interactor B | intact:EBI-2514233 uniprotkb:Q9UEN0 uniprotkb:Q9H3T6 uniprotkb:O76026 uniprotkb:Q9UP39 uniprotkb:B2R6U3 ensembl:ENSP00000369217.4 |
| Aliases for A | psi-mi:cul1_human(display_long) uniprotkb:CUL1(gene name) psi-mi:CUL1(display_short) |
| Aliases for B | psi-mi:ari1_human(display_long) uniprotkb:Protein ariadne-1 homolog(gene name synonym) uniprotkb:ARIH1(gene name) psi-mi:ARIH1(display_short) uniprotkb:ARI(gene name synonym) uniprotkb:MOP6(gene name synonym) uniprotkb:UBCH7BP(gene name synonym) uniprotkb:Ubiquitin-conjugating enzyme E2-binding protein 1(gene name synonym) uniprotkb:UbcH7-binding protein(gene name synonym) uniprotkb:UbcM4-interacting protein(gene name synonym) uniprotkb:HHARI(gene name synonym) uniprotkb:H7-AP2(gene name synonym) uniprotkb:Monocyte protein 6(gene name synonym) uniprotkb:HUSSY-27(orf name) |
| Interaction detection methods | psi-mi:"MI:0007"(anti tag coimmunoprecipitation) |
| First author | Kelsall et al. (2013) |
| Identifier of the publication | imex:IM-22207 pubmed:24076655 |
| NCBI Taxonomy identifier for interactor A | taxid:9606(human) taxid:9606(Homo sapiens) |
| NCBI Taxonomy identifier for interactor B | taxid:9606(human) taxid:9606(Homo sapiens) |
| Interaction types | psi-mi:"MI:0915"(physical association) |
| Source databases and identifiers | psi-mi:"MI:0471"(MINT) |
| Interaction identifier(s) in the corresponding source database | intact:EBI-9118068 imex:IM-22207-14 |
| Confidence score | intact-miscore:0.77 |
| Complex expansion | - |
| Biological role A | Unspecified role |
| Biological role B | Unspecified role |
| Experimental role A | Prey |
| Experimental role B | Bait |
| Interactor type A | Protein |
| Interactor type B | Protein |
| Annotations for the interaction | figure legend:f5c f5b comment:"\"Precipitation of GFP‐HHARI with either an N‐terminal 100‐aa deletion (GFP‐HHARI (aa 101–557)) or triple mutations in the predicted UBA‐like domain (V123A, I124A, and W140A) abolished or significantly reduced the interaction with CUL1‐NEDD8 (Figure 5B and C), whereas the RING1 H205A mutation significantly decreased UBCH7 binding without disrupting the interaction with CUL1‐NEDD8.\"" curation depth:imex curation full coverage:Only protein-protein interactions |
| NCBI Taxonomy identifier for the host organism | taxid:9606(human-293) taxid:9606(Homo sapiens HEK293 embryonic kidney cell) |
| Parameters of the interaction | - |
| Creation date | 2014/02/04 |
| Update date | 2025/03/31 |
| negative Boolean value | false |
| Feature(s) for interactor A | - |
| Feature(s) for interactor B | green fluorescent protein tag:?-? binding-associated region:1-100 mutation disrupting interaction:123-123,124-124,140-140 |
| Stoichiometry for interactor A | - |
| Stoichiometry for interactor B | - |
| Participant identification method for interactor A | Western blot |
| Participant identification method for interactor B | Anti tag western blot |