| Unique identifier for interactor A | uniprotkb:O94829 |
| Unique identifier for interactor B | uniprotkb:P61326 |
| Alternative identifier for interactor A | intact:EBI-747310 uniprotkb:D3DPY4 ensembl:ENSP00000361418.3 uniprotkb:Q7LC04 uniprotkb:Q96HS3 uniprotkb:Q9H8N3 uniprotkb:Q9UFR1 uniprotkb:Q5T4X3 |
| Alternative identifier for interactor B | intact:EBI-299134 ensembl:ENSP00000360525.3 uniprotkb:B1ARP8 uniprotkb:O35169 uniprotkb:P50606 uniprotkb:Q5SW69 uniprotkb:B2R5A2 |
| Aliases for A | psi-mi:ipo13_human(display_long) uniprotkb:Ran-binding protein 13(gene name synonym) uniprotkb:Karyopherin-13(gene name synonym) uniprotkb:IPO13(gene name) psi-mi:IPO13(display_short) uniprotkb:KIAA0724(gene name synonym) uniprotkb:RANBP13(gene name synonym) |
| Aliases for B | psi-mi:mgn_human(display_long) uniprotkb:MAGOH(gene name) psi-mi:MAGOH(display_short) uniprotkb:MAGOHA(gene name synonym) |
| Interaction detection methods | psi-mi:"MI:1235"(thermal shift binding) |
| First author | Grünwald et al. (2013) |
| Identifier of the publication | pubmed:23435562 imex:IM-22198 |
| NCBI Taxonomy identifier for interactor A | taxid:9606(human) taxid:9606(Homo sapiens) |
| NCBI Taxonomy identifier for interactor B | taxid:9606(human) taxid:9606(Homo sapiens) |
| Interaction types | psi-mi:"MI:0915"(physical association) |
| Source databases and identifiers | psi-mi:"MI:0471"(MINT) |
| Interaction identifier(s) in the corresponding source database | intact:EBI-9208046 imex:IM-22198-15 |
| Confidence score | intact-miscore:0.67 |
| Complex expansion | psi-mi:"MI:1060"(spoke expansion) |
| Biological role A | Unspecified role |
| Biological role B | Unspecified role |
| Experimental role A | Neutral component |
| Experimental role B | Neutral component |
| Interactor type A | Protein |
| Interactor type B | Protein |
| Annotations for the interaction | figure legend:f3d sf3 comment:"\"To better understand the mechanism of the Imp13 import/export cycle, we measured the KD values by differential scanning fluorimetry (DSF) (Niesen et al, 2007) (Figure 3D; Supplementary Figure 3). The estimated KD for the two import cargoes Mago-Y14 and Ubc9 is in a similar range with a value of 235±30nM and 370±20 nM, respectively. The KD value for RanGTP binding to Imp13, although not accurately calculated due to the upper detection limit of the method, falls in the low nanomolar or picomolar range (o100 nM) and is consistent with values measured for other karyopherins (Bischoff and Go¨rlich, 1997; Deane et al, 1997; Go¨ rlich et al, 1997; Lipowsky et al, 2000). The dissociation constant of the export cargo eIF1A to a preformed Imp13-RanGTP complex is 3±0.38 mM, 10-fold higher than the KD values of the import cargoes (Figure 3D).\"" full coverage:Only protein-protein interactions curation depth:imex curation |
| NCBI Taxonomy identifier for the host organism | taxid:-1(in vitro) taxid:-1(In vitro) |
| Parameters of the interaction | kd:0.23x10^-6 |
| Creation date | 2014/02/17 |
| Update date | 2014/10/16 |
| negative Boolean value | false |
| Feature(s) for interactor A | - |
| Feature(s) for interactor B | - |
| Stoichiometry for interactor A | - |
| Stoichiometry for interactor B | - |
| Participant identification method for interactor A | Predetermined participant |
| Participant identification method for interactor B | Predetermined participant |