Curation Details
Interaction ID: IM-22191-1

Unique identifier for interactor Auniprotkb:Q15393
Unique identifier for interactor Buniprotkb:Q04637
Alternative identifier for interactor Aintact:EBI-346977
uniprotkb:Q6NTI8
uniprotkb:Q96GC0
uniprotkb:Q9BPY2
uniprotkb:Q9UFX7
uniprotkb:Q9UJ29
ensembl:ENSP00000305790.5
Alternative identifier for interactor Bintact:EBI-73711
uniprotkb:O43177
uniprotkb:O95066
uniprotkb:Q5HYG0
uniprotkb:Q6ZN21
uniprotkb:Q8N102
intact:EBI-11020102
uniprotkb:D3DNT4
uniprotkb:D3DNT5
uniprotkb:D3DNT2
uniprotkb:E9PFM1
uniprotkb:G5E9S1
ensembl:ENSP00000316879.5
intact:EBI-28974642
Aliases for Apsi-mi:sf3b3_human(display_long)
uniprotkb:SF3B3(gene name)
psi-mi:SF3B3(display_short)
uniprotkb:KIAA0017(gene name synonym)
uniprotkb:SAP130(gene name synonym)
uniprotkb:Spliceosome-associated protein 130(gene name synonym)
uniprotkb:Pre-mRNA-splicing factor SF3b 130 kDa subunit(gene name synonym)
uniprotkb:STAF130(gene name synonym)
Aliases for Bpsi-mi:if4g1_human(display_long)
uniprotkb:p220(gene name synonym)
uniprotkb:EIF4G1(gene name)
psi-mi:EIF4G1(display_short)
uniprotkb:EIF4G(gene name synonym)
uniprotkb:EIF4GI(gene name synonym)
uniprotkb:EIF4F(gene name synonym)
Interaction detection methodspsi-mi:"MI:0006"(anti bait coimmunoprecipitation)
First authorGumireddy et al. (2013)
Identifier of the publicationpubmed:23974796
imex:IM-22191
NCBI Taxonomy identifier for interactor Ataxid:9606(human)
taxid:9606(Homo sapiens)
NCBI Taxonomy identifier for interactor Btaxid:9606(human)
taxid:9606(Homo sapiens)
Interaction typespsi-mi:"MI:0914"(association)
Source databases and identifierspsi-mi:"MI:0471"(MINT)
Interaction identifier(s) in the corresponding source databaseintact:EBI-9086436
imex:IM-22191-1
Confidence scoreintact-miscore:0.35
Complex expansionpsi-mi:"MI:1060"(spoke expansion)
Biological role AUnspecified role
Biological role BUnspecified role
Experimental role ABait
Experimental role BPrey
Interactor type AProtein
Interactor type BProtein
Annotations for the interactionfigure legend:f5b
comment:"\"To investigate whether treRNA expression promoted RNP complex formation and the binding of eIF4G1 to the complex, we immunoprecipitated SF3B3 or eIF4G1 in MCF7 cells expressing treRNA or a vector control or a control lncRNA HAR1A. Co‐immunoprecipitation experiments showed that SF3B3 was associated with PUF60 in MCF7 cells expressing treRNA or a vector control or HAR1A (Figure 5B), confirming the results from previous publications that splicing factor 3B protein complex binds to PUF60 (Corsini et al, 2009; Prigge et al, 2009). Interestingly, SF3B3 interacted with hnRNP K, FXR1, FXR2, and eIF4G1 in MCF7 cells expressing treRNA, but not in MCF7 cells expressing a vector control or HAR1A, demonstrating that treRNA was required for the formation of treRNA RNP complex and its binding to eIF4G1 (Figure 5B).\""
curation depth:imex curation
full coverage:Only protein-protein interactions
NCBI Taxonomy identifier for the host organismtaxid:9606(human-mcf7)
taxid:9606(Homo sapiens MCF-7 mammary adenocarcinoma cell)
Parameters of the interaction-
Creation date2014/01/16
Update date2025/12/29
negative Boolean valuefalse
Feature(s) for interactor A-
Feature(s) for interactor B-
Stoichiometry for interactor A-
Stoichiometry for interactor B-
Participant identification method for interactor APredetermined participant
Participant identification method for interactor BWestern blot