| Unique identifier for interactor A | uniprotkb:Q9UQB8-4 |
| Unique identifier for interactor B | uniprotkb:P50552 |
| Alternative identifier for interactor A | intact:EBI-6174091 ensembl:ENSP00000315685.7 |
| Alternative identifier for interactor B | intact:EBI-748201 uniprotkb:B2RBT9 ensembl:ENSP00000245932.5 intact:EBI-28992147 uniprotkb:Q6PIZ1 uniprotkb:Q93035 |
| Aliases for A | psi-mi:q9uqb8-4(display_long) uniprotkb:BAIAP2-alpha(isoform synonym) uniprotkb:BAIAP2(gene name) psi-mi:BAIAP2(display_short) uniprotkb:Fas ligand-associated factor 3(gene name synonym) uniprotkb:Insulin receptor substrate p53/p58(gene name synonym) uniprotkb:Insulin receptor substrate protein of 53 kDa(gene name synonym) |
| Aliases for B | psi-mi:vasp_human(display_long) uniprotkb:VASP(gene name) psi-mi:VASP(display_short) |
| Interaction detection methods | psi-mi:"MI:0038"(dynamic light scattering) |
| First author | Disanza et al. (2013) |
| Identifier of the publication | imex:IM-22092 pubmed:24076653 |
| NCBI Taxonomy identifier for interactor A | taxid:9606(human) taxid:9606(Homo sapiens) |
| NCBI Taxonomy identifier for interactor B | taxid:9606(human) taxid:9606(Homo sapiens) |
| Interaction types | psi-mi:"MI:0407"(direct interaction) |
| Source databases and identifiers | psi-mi:"MI:0471"(MINT) |
| Interaction identifier(s) in the corresponding source database | intact:EBI-9201651 imex:IM-22092-4 |
| Confidence score | intact-miscore:0.65 |
| Complex expansion | - |
| Biological role A | Unspecified role |
| Biological role B | Unspecified role |
| Experimental role A | Neutral component |
| Experimental role B | Neutral component |
| Interactor type A | Protein |
| Interactor type B | Protein |
| Annotations for the interaction | figure legend:f1a comment:"\"Purified IRSp53 produced a single major species with a hydrodynamic radius of about 7.2 nm (Figure 1A) consistent with its dimeric structure (similar results were obtained by hydrodynamic measurements; Supplementary Figure S2a), while the VASP tetramer gave rise to a single species with a radius of about 14.3 nm (Figure 1A). Notably, the mixture of both proteins led to the formation of large clusters with an average diameter of about 200 nm (Figure 2A, left). Importantly, the VASP‐ΔPRD mutant, which is unable to bind to IRSp53 (Supplementary Figure S1e), and the IRSp53 W413G mutant, which lacks VASP binding ability (Supplementary Figure S1c), did not form heterocomplexes (Figure 1A, right, and data not shown). In contrast, a VASP mutant lacking the central three GP5 motifs (VASP‐ΔGP5), which retains IRSp53 binding ability, albeit with reduced affinity (Supplementary Figure S1d and f), was still able to hetero‐oligomerize upon addition of IRSp53 (Figure 1A, middle).\"" full coverage:Only protein-protein interactions curation depth:imex curation |
| NCBI Taxonomy identifier for the host organism | taxid:-1(in vitro) taxid:-1(In vitro) |
| Parameters of the interaction | - |
| Creation date | 2014/02/04 |
| Update date | 2014/10/16 |
| negative Boolean value | false |
| Feature(s) for interactor A | - |
| Feature(s) for interactor B | binding-associated region:162-206 |
| Stoichiometry for interactor A | - |
| Stoichiometry for interactor B | - |
| Participant identification method for interactor A | Predetermined participant |
| Participant identification method for interactor B | Predetermined participant |