| Unique identifier for interactor A | uniprotkb:P22888 |
| Unique identifier for interactor B | uniprotkb:P22888 |
| Alternative identifier for interactor A | intact:EBI-9084084 uniprotkb:Q14751 uniprotkb:Q15996 uniprotkb:Q9UEW9 ensembl:ENSP00000294954.6 |
| Alternative identifier for interactor B | intact:EBI-9084084 uniprotkb:Q14751 uniprotkb:Q15996 uniprotkb:Q9UEW9 ensembl:ENSP00000294954.6 |
| Aliases for A | psi-mi:lshr_human(display_long) uniprotkb:LHCGR(gene name) psi-mi:LHCGR(display_short) uniprotkb:LCGR(gene name synonym) uniprotkb:LGR2(gene name synonym) uniprotkb:LHRHR(gene name synonym) uniprotkb:Luteinizing hormone receptor(gene name synonym) |
| Aliases for B | psi-mi:lshr_human(display_long) uniprotkb:LHCGR(gene name) psi-mi:LHCGR(display_short) uniprotkb:LCGR(gene name synonym) uniprotkb:LGR2(gene name synonym) uniprotkb:LHRHR(gene name synonym) uniprotkb:Luteinizing hormone receptor(gene name synonym) |
| Interaction detection methods | psi-mi:"MI:0052"(fluorescence correlation spectroscopy) |
| First author | Grzesik et al. (2014) |
| Identifier of the publication | pubmed:24438591 imex:IM-22077 |
| NCBI Taxonomy identifier for interactor A | taxid:9606(human) taxid:9606(Homo sapiens) |
| NCBI Taxonomy identifier for interactor B | taxid:9606(human) taxid:9606(Homo sapiens) |
| Interaction types | psi-mi:"MI:0915"(physical association) |
| Source databases and identifiers | psi-mi:"MI:0471"(MINT) |
| Interaction identifier(s) in the corresponding source database | intact:EBI-9084082 imex:IM-22077-1 |
| Confidence score | intact-miscore:0.46 |
| Complex expansion | - |
| Biological role A | Unspecified role |
| Biological role B | Unspecified role |
| Experimental role A | Neutral component |
| Experimental role B | Neutral component |
| Interactor type A | Protein |
| Interactor type B | Protein |
| Annotations for the interaction | figure legend:f3 comment:"\"Using fluorescent cross-correlation spectroscopy (FCCS), we tested each co-expressed pair of signaling- and binding-deficient mutants for significant cross-correlation. As a positive control, we used wild type hLHR, and LHR-homodimerization was indeed confirmed in our FCCS experiments. As a negative control, we used a membrane-attached, A kinase anchor protein 18 alpha (AKAP18α-mCherry) construct, co-transfected with hLHR-delExon10. A cross-correlation of 7% was determined, which is consistent with the cross-talk of the systemand indicates that the molecules do not interact. For each pair of deficient mutants, including the hLHR-delExon10, there was significant cross-correlation at the basal cell membrane. Figures 3B and 3C show representative, normalized auto- and cross-correlation curves of GFP, mCherry-tagged receptor constructs hLHR-delExon10, mutant hLHR-C131R, and the negative control with the AKAP18α construct. It was observed that neither the introduced point mutations, such as Cys131Arg and Lys605Glu, nor the complete lack of Exon10 has a significant influence on receptor oligomerization (figure 3A). These results confirm that oligomerization of the hLHRdelExon10 is not disturbed by the lack of the exon10.\"" full coverage:Only protein-protein interactions curation depth:imex curation |
| NCBI Taxonomy identifier for the host organism | taxid:9606(human-293) taxid:9606(Homo sapiens HEK293 embryonic kidney cell) |
| Parameters of the interaction | - |
| Creation date | 2014/01/14 |
| Update date | 2014/10/16 |
| negative Boolean value | false |
| Feature(s) for interactor A | green fluorescent protein tag:?-? |
| Feature(s) for interactor B | mcherry fluorescent protein tag:?-? |
| Stoichiometry for interactor A | - |
| Stoichiometry for interactor B | - |
| Participant identification method for interactor A | Tag visualisation by fluorescence |
| Participant identification method for interactor B | Tag visualisation by fluorescence |