| Unique identifier for interactor A | uniprotkb:P25963 |
| Unique identifier for interactor B | uniprotkb:P23396 |
| Alternative identifier for interactor A | intact:EBI-307386 uniprotkb:B2R8L6 ensembl:ENSP00000216797.6 |
| Alternative identifier for interactor B | intact:EBI-351193 uniprotkb:Q498B5 uniprotkb:Q8NI95 uniprotkb:B2R7N5 uniprotkb:J3KN86 ensembl:ENSP00000433821.1 ensembl:ENSP00000434643.1 ensembl:ENSP00000436971.1 |
| Aliases for A | psi-mi:ikba_human(display_long) uniprotkb:MAD3(gene name synonym) uniprotkb:NFKBIA(gene name) psi-mi:NFKBIA(display_short) uniprotkb:IKBA(gene name synonym) uniprotkb:NFKBI(gene name synonym) uniprotkb:I-kappa-B-alpha(gene name synonym) uniprotkb:Major histocompatibility complex enhancer-binding protein MAD3(gene name synonym) |
| Aliases for B | psi-mi:rs3_human(display_long) uniprotkb:RPS3(gene name) psi-mi:RPS3(display_short) uniprotkb:40S ribosomal protein S3(gene name synonym) uniprotkb:OK/SW-cl.26(orf name) |
| Interaction detection methods | psi-mi:"MI:0096"(pull down) |
| First author | Stanborough et al. (2014) |
| Identifier of the publication | pubmed:24457201 imex:IM-22074 |
| NCBI Taxonomy identifier for interactor A | taxid:9606(human) taxid:9606(Homo sapiens) |
| NCBI Taxonomy identifier for interactor B | taxid:9606(human) taxid:9606(Homo sapiens) |
| Interaction types | psi-mi:"MI:0915"(physical association) |
| Source databases and identifiers | psi-mi:"MI:0471"(MINT) |
| Interaction identifier(s) in the corresponding source database | intact:EBI-9081581 imex:IM-22074-5 |
| Confidence score | intact-miscore:0.68 |
| Complex expansion | psi-mi:"MI:1060"(spoke expansion) |
| Biological role A | Unspecified role |
| Biological role B | Unspecified role |
| Experimental role A | Neutral component |
| Experimental role B | Neutral component |
| Interactor type A | Protein |
| Interactor type B | Protein |
| Annotations for the interaction | figure legend:f4b comment:"\"The Flag-IκBα-6xHis-RPS3 complex was purified via His-tag purification of 6xHis-RPS3 followed by binding of the His-eluate to ANTIFLAG ® M2 affinity gel (Sigma). The immobilized Flag-IκBα-6xHis-RPS3 complex as well as anti-FLAG agarose bound Flag-IκBα and anti-FLAG agarose bound Flag- RPS3 were incubated with an approximately three-fold molar excess of p65 or with buffer containing TEV-protease (control). Following washing of the beads in washing buffer, bound proteins were eluted by competition with FLAG Peptide (Sigma).\"" curation depth:imex curation full coverage:Only protein-protein interactions |
| NCBI Taxonomy identifier for the host organism | taxid:-1(in vitro) taxid:-1(In vitro) |
| Parameters of the interaction | - |
| Creation date | 2014/01/11 |
| Update date | 2024/11/30 |
| negative Boolean value | false |
| Feature(s) for interactor A | flag tag:?-? |
| Feature(s) for interactor B | his tag:?-? |
| Stoichiometry for interactor A | - |
| Stoichiometry for interactor B | - |
| Participant identification method for interactor A | Anti tag western blot |
| Participant identification method for interactor B | Anti tag western blot |