| Unique identifier for interactor A | uniprotkb:Q9BTE3 |
| Unique identifier for interactor B | uniprotkb:P33993 |
| Alternative identifier for interactor A | intact:EBI-749378 uniprotkb:B3KSP7 ensembl:ENSP00000353098.3 uniprotkb:Q6IA56 uniprotkb:Q9BVT9 uniprotkb:Q9H916 |
| Alternative identifier for interactor B | intact:EBI-355924 uniprotkb:A4D2A1 uniprotkb:A4D2A2 uniprotkb:E9PGN9 ensembl:ENSP00000307288.5 uniprotkb:Q15076 uniprotkb:Q96D34 uniprotkb:Q96GL1 |
| Aliases for A | psi-mi:mcmbp_human(display_long) uniprotkb:MCMBP(gene name) psi-mi:MCMBP(display_short) uniprotkb:C10orf119(gene name synonym) |
| Aliases for B | psi-mi:mcm7_human(display_long) uniprotkb:MCM7(gene name) psi-mi:MCM7(display_short) uniprotkb:CDC47(gene name synonym) uniprotkb:MCM2(gene name synonym) uniprotkb:CDC47 homolog(gene name synonym) uniprotkb:P1.1-MCM3(gene name synonym) |
| Interaction detection methods | psi-mi:"MI:0007"(anti tag coimmunoprecipitation) |
| First author | Kusunoki et al. (2013) |
| Identifier of the publication | pubmed:24299456 imex:IM-22017 |
| NCBI Taxonomy identifier for interactor A | taxid:9606(human) taxid:9606(Homo sapiens) |
| NCBI Taxonomy identifier for interactor B | taxid:9606(human) taxid:9606(Homo sapiens) |
| Interaction types | psi-mi:"MI:0914"(association) |
| Source databases and identifiers | psi-mi:"MI:0471"(MINT) |
| Interaction identifier(s) in the corresponding source database | intact:EBI-8875990 imex:IM-22017-5 |
| Confidence score | intact-miscore:0.90 |
| Complex expansion | psi-mi:"MI:1060"(spoke expansion) |
| Biological role A | Unspecified role |
| Biological role B | Unspecified role |
| Experimental role A | Bait |
| Experimental role B | Prey |
| Interactor type A | Protein |
| Interactor type B | Protein |
| Annotations for the interaction | figure legend:f5 comment:"\"To understand the interaction of MCM2-7 with MCM-BP at protein level, following experiment was carried out. Flag-tagged MCM-BP was co-expressed with MCM2-7 proteins in insect cells. Flag-tagged MCM-BP was purified using anti-Flag-agarose beads, and co-purified MCM2-7 proteins were analyzed (Fig. 5). Purified protein complex containing Flag-MCM-BP was separated by glycerol gradient centrifugation. As reported earlier (20), co-recovery of MCM3-7 was observed. Western-blot analysis where purified MCM2-7 proteins were co-electrophoresed shows that MCM3, MCM5 and MCM7 were mainly recovered with MCM-BP and smaller amounts of MCM4 and MCM6 were also recovered, suggesting that MCM-BP has higher affinity for MCM3, MCM5 and MCM7. Position of sedimentation suggests that MCM-BP mainly form dimmers by interacting with MCM3, MCM5 or MCM7. Thus these all results suggest that MCM-BP disassembles MCM2-7 complex on the DNA by interacting with MCM3, MCM5 and MCM7 proteins.\"" full coverage:Only protein-protein interactions curation depth:imex curation |
| NCBI Taxonomy identifier for the host organism | taxid:7111(trini-high_5) taxid:7111(Trichoplusia ni cell line from eggs) |
| Parameters of the interaction | - |
| Creation date | 2013/11/27 |
| Update date | 2014/10/16 |
| negative Boolean value | false |
| Feature(s) for interactor A | flag tag:?-? |
| Feature(s) for interactor B | his tag:?-? |
| Stoichiometry for interactor A | - |
| Stoichiometry for interactor B | - |
| Participant identification method for interactor A | Western blot |
| Participant identification method for interactor B | Western blot |