| Unique identifier for interactor A | uniprotkb:Q9BTE3 |
| Unique identifier for interactor B | uniprotkb:Q14566 |
| Alternative identifier for interactor A | intact:EBI-749378 uniprotkb:Q6IA56 uniprotkb:Q9BVT9 uniprotkb:Q9H916 uniprotkb:B3KSP7 ensembl:ENSP00000353098.3 |
| Alternative identifier for interactor B | intact:EBI-374900 uniprotkb:Q13504 uniprotkb:Q99859 uniprotkb:B2R6H2 ensembl:ENSP00000264156.2 |
| Aliases for A | psi-mi:mcmbp_human(display_long) uniprotkb:MCMBP(gene name) psi-mi:MCMBP(display_short) uniprotkb:C10orf119(gene name synonym) |
| Aliases for B | psi-mi:mcm6_human(display_long) uniprotkb:MCM6(gene name) psi-mi:MCM6(display_short) uniprotkb:p105MCM(gene name synonym) |
| Interaction detection methods | psi-mi:"MI:0006"(anti bait coimmunoprecipitation) |
| First author | Kusunoki et al. (2013) |
| Identifier of the publication | pubmed:24299456 imex:IM-22017 |
| NCBI Taxonomy identifier for interactor A | taxid:9606(human) taxid:9606(Homo sapiens) |
| NCBI Taxonomy identifier for interactor B | taxid:9606(human) taxid:9606(Homo sapiens) |
| Interaction types | psi-mi:"MI:0914"(association) |
| Source databases and identifiers | psi-mi:"MI:0471"(MINT) |
| Interaction identifier(s) in the corresponding source database | intact:EBI-8875903 imex:IM-22017-4 |
| Confidence score | intact-miscore:0.85 |
| Complex expansion | psi-mi:"MI:1060"(spoke expansion) |
| Biological role A | Unspecified role |
| Biological role B | Unspecified role |
| Experimental role A | Bait |
| Experimental role B | Prey |
| Interactor type A | Protein |
| Interactor type B | Protein |
| Annotations for the interaction | figure legend:f2 comment:"\"Cell lysate was prepared from unsynchronized HeLa cells in the presence of 100 mM NaCl, and MCM-BP in Triton-soluble fraction was immuno-precipitated with anti-MCM-BP antibody bound to protein G (Fig. 2A). With precipitation of MCM-BP, co-precipitation of MCM3, MCM4, MCM5, MCM6 and MCM7 was detected, and these results are consistent with those reported earlier (20). In addition to MCM3-7, co-precipitation of SMC3 and ORC3 was newly detected. These results are essentially consistent with those obtained by immuno-precipitation using the co-expressed insect cell lysate. Thus it is probable that these proteins directly interact with MCM-BP in HeLa cells.\"" comment:"\"First, immuno-precipitation was performed using HeLa cells at early S phase (Fig. 2B). With precipitation of MCM-BP, co-precipitation of MCM5 and MCM7 was detected. Faint bands of MCM4 and SMC3 proteins were detected in the precipitate. Next, immuno-precipitation was carried out using lysate of HeLa cells synchronized at G2 phase (Fig. 2C). With precipitation of MCM-BP, co-precipitation of MCM7 and SMC3 was detected. Faint band of MCM5 was detected.\"" curation depth:imex curation full coverage:Only protein-protein interactions |
| NCBI Taxonomy identifier for the host organism | taxid:9606(human-hela) taxid:9606(Homo sapiens HeLa epitheloid cervical carcinoma cell) |
| Parameters of the interaction | - |
| Creation date | 2013/11/27 |
| Update date | 2024/09/12 |
| negative Boolean value | false |
| Feature(s) for interactor A | - |
| Feature(s) for interactor B | - |
| Stoichiometry for interactor A | - |
| Stoichiometry for interactor B | - |
| Participant identification method for interactor A | Western blot |
| Participant identification method for interactor B | Western blot |