| Unique identifier for interactor A | uniprotkb:P43403 |
| Unique identifier for interactor B | uniprotkb:Q8BGG7 |
| Alternative identifier for interactor A | intact:EBI-1211276 ensembl:ENSP00000264972.5 ensembl:ENSP00000513759.1 ensembl:ENSP00000517108.1 ensembl:ENSP00000517109.1 uniprotkb:A6NFP4 uniprotkb:Q6PIA4 uniprotkb:Q8IXD6 uniprotkb:Q9UBS6 |
| Alternative identifier for interactor B | intact:EBI-8846415 uniprotkb:Q3U8Z2 uniprotkb:Q8BMW9 ensembl:ENSMUSP00000043865.9 |
| Aliases for A | psi-mi:zap70_human(display_long) uniprotkb:ZAP70(gene name) psi-mi:ZAP70(display_short) uniprotkb:SRK(gene name synonym) uniprotkb:Syk-related tyrosine kinase(gene name synonym) uniprotkb:70 kDa zeta-chain associated protein(gene name synonym) |
| Aliases for B | psi-mi:ubs3b_mouse(display_long) uniprotkb:Ubash3b(gene name) psi-mi:Ubash3b(display_short) uniprotkb:Sts1(gene name synonym) uniprotkb:Cbl-interacting protein p70(gene name synonym) uniprotkb:Suppressor of T-cell receptor signaling 1(gene name synonym) uniprotkb:T-cell ubiquitin ligand 2(gene name synonym) uniprotkb:Tyrosine-protein phosphatase STS1/TULA2(gene name synonym) |
| Interaction detection methods | psi-mi:"MI:0096"(pull down) |
| First author | Luis et al. (2013) |
| Identifier of the publication | pubmed:24256567 imex:IM-21762 |
| NCBI Taxonomy identifier for interactor A | taxid:9606(human) taxid:9606(Homo sapiens) |
| NCBI Taxonomy identifier for interactor B | taxid:10090(mouse) taxid:10090(Mus musculus) |
| Interaction types | psi-mi:"MI:0915"(physical association) |
| Source databases and identifiers | psi-mi:"MI:0471"(MINT) |
| Interaction identifier(s) in the corresponding source database | intact:EBI-8846412 imex:IM-21762-1 |
| Confidence score | intact-miscore:0.59 |
| Complex expansion | - |
| Biological role A | Unspecified role |
| Biological role B | Unspecified role |
| Experimental role A | Bait |
| Experimental role B | Prey |
| Interactor type A | Protein |
| Interactor type B | Protein |
| Annotations for the interaction | figure legend:f1c comment:"\"We began by assessing the interaction between a Zap-70-derived phosphopeptide (GSVYESPpYSDPEEL) and the different Sts-1PGM mutants described above. Pulldown assays were performed in which phosphopeptide-coupled beads were added to lysates prepared from cells expressing wild-type or mutant Sts-1PGM, and bound proteins were assessed by immunoblot analysis. We observed that four Sts-1PGM mutants interacted stably with the bound phosphopeptide, asevidenced by their ability to be isolated from the lysate (Figure 1C). Interestingly, the four mutants appeared to interact with the phospho-peptide beads with substantially different affinities. Specifically, Sts-1PGM H380C single mutant exhibited very weak interaction with the phospho-peptide beads, while the double mutants Sts-1PGM H380C/E490A and Sts-1PGM H380C/E490Q appeared to have the strongest affinity and the Sts-1PGM H380C/R462A/E490Q triple mutant was precipitated to an intermediate degree. Full-length Sts-1 variants also displayed similar binding characteristics (data not shown), with double mutants H380C/E490A and H380C/E490Q (henceforth referred to as CA and CQ respectively) displaying increased binding capabilities than the single mutant H380C (henceforth referred to as C). These results suggest that the CA and CQ mutants could serve as significantly better substrate-trapping enzymes than the C mutant.\"" full coverage:Only protein-protein interactions curation depth:imex curation |
| NCBI Taxonomy identifier for the host organism | taxid:-1(in vitro) taxid:-1(In vitro) |
| Parameters of the interaction | - |
| Creation date | 2013/11/08 |
| Update date | 2014/10/16 |
| negative Boolean value | false |
| Feature(s) for interactor A | binding-associated region:312-325 porphyrin modified residue:319-319 |
| Feature(s) for interactor B | flag tag:?-? binding-associated region:369-638 trapping mutant:380-380 |
| Stoichiometry for interactor A | - |
| Stoichiometry for interactor B | - |
| Participant identification method for interactor A | Predetermined participant |
| Participant identification method for interactor B | Anti tag western blot |