| Unique identifier for interactor A | uniprotkb:P42224 |
| Unique identifier for interactor B | uniprotkb:O60674 |
| Alternative identifier for interactor A | intact:EBI-1057697 uniprotkb:Q53XW4 uniprotkb:B2RCA0 uniprotkb:Q9UDL5 uniprotkb:A8K989 uniprotkb:D2KFR8 uniprotkb:Q68D00 uniprotkb:D3DPI7 ensembl:ENSP00000354394.4 ensembl:ENSP00000386244.1 ensembl:ENSP00000388240.2 ensembl:ENSP00000438703.2 ensembl:ENSP00000513582.1 uniprotkb:Q53S88 |
| Alternative identifier for interactor B | intact:EBI-518647 ensembl:ENSP00000371067.4 uniprotkb:O14636 uniprotkb:O75297 intact:EBI-28977715 |
| Aliases for A | psi-mi:stat1_human(display_long) uniprotkb:STAT1(gene name) psi-mi:STAT1(display_short) uniprotkb:Transcription factor ISGF-3 components p91/p84(gene name synonym) |
| Aliases for B | psi-mi:jak2_human(display_long) uniprotkb:Janus kinase 2(gene name synonym) uniprotkb:JAK2(gene name) psi-mi:JAK2(display_short) |
| Interaction detection methods | psi-mi:"MI:0424"(protein kinase assay) |
| First author | Menegazzi et al. (2013) |
| Identifier of the publication | pubmed:24255956 imex:IM-21760 |
| NCBI Taxonomy identifier for interactor A | taxid:9606(human) taxid:9606(Homo sapiens) |
| NCBI Taxonomy identifier for interactor B | taxid:9606(human) taxid:9606(Homo sapiens) |
| Interaction types | psi-mi:"MI:0217"(phosphorylation reaction) |
| Source databases and identifiers | psi-mi:"MI:0471"(MINT) |
| Interaction identifier(s) in the corresponding source database | intact:EBI-8843412 imex:IM-21760-1 |
| Confidence score | intact-miscore:0.44 |
| Complex expansion | - |
| Biological role A | Enzyme target |
| Biological role B | Enzyme |
| Experimental role A | Neutral component |
| Experimental role B | Neutral component |
| Interactor type A | Protein |
| Interactor type B | Protein |
| Annotations for the interaction | figure legend:f5 sf3 comment:"\"To demonstrate that the direct interaction occurring between active catechins and STAT1 is one of the principal molecular mechanisms of STAT1 Tyr701 phosphorylation inhibition, a cell-free kinase in vitro assay was performed. Recombinant STAT1, pre-incubated with active catechins, were mixed with recombinant active JAK2. Western blot shows the ability of anti-STAT1 catechins to reduce, in a dose-dependent manner, in vitro STAT1 phosphorylation (Figure 5). In line with our hypothesis, we also demonstrated that the inactive compounds were not able to prevent STAT1 phosphorylation (data not shown).\"" comment:Finally, cell free JAK2-kinase assays support SPR and docking data, and confirm that the direct binding of active catechins with STAT1 protein is an important event to affect Tyr701 phosphorylation. Indeed, in vitro kinase assay data indicate that wtSTAT1-catechins pre-treatmentwas able to inhibit, in a dose dependent manner, STAT1 Tyr701 phosphorylation otherwise these effect was not observed with H568A-STAT1 protein full coverage:Only protein-protein interactions curation depth:imex curation |
| NCBI Taxonomy identifier for the host organism | taxid:-1(in vitro) taxid:-1(In vitro) |
| Parameters of the interaction | - |
| Creation date | 2013/11/05 |
| Update date | 2014/10/16 |
| negative Boolean value | false |
| Feature(s) for interactor A | phosphorylated residue:701-701 |
| Feature(s) for interactor B | N-phosphorylated residue:1008-1008 |
| Stoichiometry for interactor A | - |
| Stoichiometry for interactor B | - |
| Participant identification method for interactor A | Western blot |
| Participant identification method for interactor B | Western blot |