| Unique identifier for interactor A | uniprotkb:Q13342 |
| Unique identifier for interactor B | uniprotkb:Q13526 |
| Alternative identifier for interactor A | intact:EBI-2865100 uniprotkb:Q92881 uniprotkb:Q13341 uniprotkb:Q4ZG66 uniprotkb:Q96TG3 uniprotkb:Q53TG1 uniprotkb:Q6NSG4 uniprotkb:E7ESH9 uniprotkb:E7EUR5 uniprotkb:E9PFJ6 uniprotkb:Q0VGE5 uniprotkb:Q3KR17 intact:EBI-28968654 ensembl:ENSP00000375899.3 |
| Alternative identifier for interactor B | intact:EBI-714158 uniprotkb:A8K4V9 ensembl:ENSP00000247970.5 ensembl:ENSP00000466962.1 uniprotkb:Q53X75 |
| Aliases for A | psi-mi:sp140_human(display_long) uniprotkb:SP140(gene name) psi-mi:SP140(display_short) uniprotkb:LYSP100(gene name synonym) uniprotkb:Lymphoid-restricted homolog of Sp100(gene name synonym) uniprotkb:Nuclear autoantigen Sp-140(gene name synonym) uniprotkb:Speckled 140 kDa(gene name synonym) |
| Aliases for B | psi-mi:pin1_human(display_long) uniprotkb:Rotamase Pin1(gene name synonym) uniprotkb:Peptidyl-prolyl cis-trans isomerase Pin1(gene name synonym) uniprotkb:PIN1(gene name) psi-mi:PIN1(display_short) |
| Interaction detection methods | psi-mi:"MI:0077"(nuclear magnetic resonance) |
| First author | Zucchelli et al. (2013) |
| Identifier of the publication | pubmed:24267382 imex:IM-21758 |
| NCBI Taxonomy identifier for interactor A | taxid:9606(human) taxid:9606(Homo sapiens) |
| NCBI Taxonomy identifier for interactor B | taxid:9606(human) taxid:9606(Homo sapiens) |
| Interaction types | psi-mi:"MI:0407"(direct interaction) |
| Source databases and identifiers | psi-mi:"MI:0471"(MINT) |
| Interaction identifier(s) in the corresponding source database | intact:EBI-8830127 imex:IM-21758-2 |
| Confidence score | intact-miscore:0.61 |
| Complex expansion | - |
| Biological role A | Unspecified role |
| Biological role B | Unspecified role |
| Experimental role A | Unspecified role |
| Experimental role B | Unspecified role |
| Interactor type A | Protein |
| Interactor type B | Protein |
| Annotations for the interaction | figure legend:f2c f2d f2e sf8a comment:"\"We next determined the binding site of EAERpTPWN on Pin1, performing NMR based chemical shift mapping assays. To this end 2D 1H-15N HSQC spectra of full-length 15Nlabeled Pin1 were recorded to monitor possible changes in Pin1 1H-15N chemical shifts upon successive additions of unlabelled peptides. A comparison of the spectra in the absence and presence of a five-fold excess (1 mM) of Sp140-peptides showed that only EAERpTPWN was able to bind Pin1, as revealed by the numerous peaks' displacements observed upon addition of the phosphopeptide (Fig. 2C, Fig. S8A). The unphosphorylated peptide did not show any binding evidence, confirming the phospho-dependence of the Pin1-peptide interaction (Fig. S8B). The complex between Pin1 and EAERpTPWN was in the fast exchange regime on the NMR chemical-shift time scale (Fig. 2C) with a dissociation constant of 138 ± 4 μM (Fig. 2D). Pin1 residues exhibiting significant amide chemical shift changes (Fig. 2E) where mapped on Pin1 crystallographic structure (pdb code: 1PIN). The binding surface mainly involved the β-sheet of the WW domain (Lys13- Ser16, Gly20, Val22-Asn26, Ala31, Gln33-Arg35) (Fig. 2F). Chemical shift changes were observed also on the flexible linker (Ser41, Ser43, Lys46) and on the region of the PPIase domain facing (Lys97-Glu100) or nearby (Phe139-Arg142) the WW domain, probably induced by long range conformational rearrangements upon complex formation. Overall, these data indicate that Pin1 binds to EAERpTPWN and catalyzes the cis-trans isomerization of its phosphothreonine-proline bond in vitro.\"" full coverage:Only protein-protein interactions curation depth:imex curation |
| NCBI Taxonomy identifier for the host organism | taxid:-1(in vitro) taxid:-1(In vitro) |
| Parameters of the interaction | kd:138.0x10^-6(molar) |
| Creation date | 2013/10/28 |
| Update date | 2014/10/16 |
| negative Boolean value | false |
| Feature(s) for interactor A | binding-associated region:722-729 phosphorylated residue:726-726 |
| Feature(s) for interactor B | his tag:?-? |
| Stoichiometry for interactor A | - |
| Stoichiometry for interactor B | - |
| Participant identification method for interactor A | Predetermined participant |
| Participant identification method for interactor B | Predetermined participant |