Curation Details
Interaction ID: IM-21758-2

Unique identifier for interactor Auniprotkb:Q13342
Unique identifier for interactor Buniprotkb:Q13526
Alternative identifier for interactor Aintact:EBI-2865100
uniprotkb:Q92881
uniprotkb:Q13341
uniprotkb:Q4ZG66
uniprotkb:Q96TG3
uniprotkb:Q53TG1
uniprotkb:Q6NSG4
ensembl:ENSP00000375899.3
uniprotkb:E7ESH9
uniprotkb:E7EUR5
uniprotkb:E9PFJ6
uniprotkb:Q0VGE5
uniprotkb:Q3KR17
intact:EBI-28968654
Alternative identifier for interactor Bintact:EBI-714158
ensembl:ENSP00000247970.5
ensembl:ENSP00000466962.1
uniprotkb:Q53X75
uniprotkb:A8K4V9
Aliases for Apsi-mi:sp140_human(display_long)
uniprotkb:SP140(gene name)
psi-mi:SP140(display_short)
uniprotkb:LYSP100(gene name synonym)
uniprotkb:Lymphoid-restricted homolog of Sp100(gene name synonym)
uniprotkb:Nuclear autoantigen Sp-140(gene name synonym)
uniprotkb:Speckled 140 kDa(gene name synonym)
Aliases for Bpsi-mi:pin1_human(display_long)
uniprotkb:Rotamase Pin1(gene name synonym)
uniprotkb:Peptidyl-prolyl cis-trans isomerase Pin1(gene name synonym)
uniprotkb:PIN1(gene name)
psi-mi:PIN1(display_short)
Interaction detection methodspsi-mi:"MI:0077"(nuclear magnetic resonance)
First authorZucchelli et al. (2013)
Identifier of the publicationpubmed:24267382
imex:IM-21758
NCBI Taxonomy identifier for interactor Ataxid:9606(human)
taxid:9606(Homo sapiens)
NCBI Taxonomy identifier for interactor Btaxid:9606(human)
taxid:9606(Homo sapiens)
Interaction typespsi-mi:"MI:0407"(direct interaction)
Source databases and identifierspsi-mi:"MI:0471"(MINT)
Interaction identifier(s) in the corresponding source databaseintact:EBI-8830127
imex:IM-21758-2
Confidence scoreintact-miscore:0.61
Complex expansion-
Biological role AUnspecified role
Biological role BUnspecified role
Experimental role AUnspecified role
Experimental role BUnspecified role
Interactor type AProtein
Interactor type BProtein
Annotations for the interactionfigure legend:f2c f2d f2e sf8a
comment:"\"We next determined the binding site of EAERpTPWN on Pin1, performing NMR based chemical shift mapping assays. To this end 2D 1H-15N HSQC spectra of full-length 15Nlabeled Pin1 were recorded to monitor possible changes in Pin1 1H-15N chemical shifts upon successive additions of unlabelled peptides. A comparison of the spectra in the absence and presence of a five-fold excess (1 mM) of Sp140-peptides showed that only EAERpTPWN was able to bind Pin1, as revealed by the numerous peaks' displacements observed upon addition of the phosphopeptide (Fig. 2C, Fig. S8A). The unphosphorylated peptide did not show any binding evidence, confirming the phospho-dependence of the Pin1-peptide interaction (Fig. S8B). The complex between Pin1 and EAERpTPWN was in the fast exchange regime on the NMR chemical-shift time scale (Fig. 2C) with a dissociation constant of 138 ± 4 μM (Fig. 2D). Pin1 residues exhibiting significant amide chemical shift changes (Fig. 2E) where mapped on Pin1 crystallographic structure (pdb code: 1PIN). The binding surface mainly involved the β-sheet of the WW domain (Lys13- Ser16, Gly20, Val22-Asn26, Ala31, Gln33-Arg35) (Fig. 2F). Chemical shift changes were observed also on the flexible linker (Ser41, Ser43, Lys46) and on the region of the PPIase domain facing (Lys97-Glu100) or nearby (Phe139-Arg142) the WW domain, probably induced by long range conformational rearrangements upon complex formation. Overall, these data indicate that Pin1 binds to EAERpTPWN and catalyzes the cis-trans isomerization of its phosphothreonine-proline bond in vitro.\""
curation depth:imex curation
full coverage:Only protein-protein interactions
NCBI Taxonomy identifier for the host organismtaxid:-1(in vitro)
taxid:-1(In vitro)
Parameters of the interactionkd:138.0x10^-6(molar)
Creation date2013/10/28
Update date2024/11/30
negative Boolean valuefalse
Feature(s) for interactor Abinding-associated region:722-729
phosphorylated residue:726-726
Feature(s) for interactor Bhis tag:?-?
Stoichiometry for interactor A-
Stoichiometry for interactor B-
Participant identification method for interactor APredetermined participant
Participant identification method for interactor BPredetermined participant