| Unique identifier for interactor A | uniprotkb:Q13342 |
| Unique identifier for interactor B | uniprotkb:Q13526 |
| Alternative identifier for interactor A | intact:EBI-2865100 uniprotkb:Q92881 uniprotkb:Q13341 uniprotkb:Q4ZG66 uniprotkb:Q96TG3 uniprotkb:Q53TG1 uniprotkb:Q6NSG4 ensembl:ENSP00000375899.3 uniprotkb:E7ESH9 uniprotkb:E7EUR5 uniprotkb:E9PFJ6 uniprotkb:Q0VGE5 uniprotkb:Q3KR17 intact:EBI-28968654 |
| Alternative identifier for interactor B | intact:EBI-714158 ensembl:ENSP00000247970.5 ensembl:ENSP00000466962.1 uniprotkb:Q53X75 uniprotkb:A8K4V9 |
| Aliases for A | psi-mi:sp140_human(display_long) uniprotkb:SP140(gene name) psi-mi:SP140(display_short) uniprotkb:LYSP100(gene name synonym) uniprotkb:Lymphoid-restricted homolog of Sp100(gene name synonym) uniprotkb:Nuclear autoantigen Sp-140(gene name synonym) uniprotkb:Speckled 140 kDa(gene name synonym) |
| Aliases for B | psi-mi:pin1_human(display_long) uniprotkb:Rotamase Pin1(gene name synonym) uniprotkb:Peptidyl-prolyl cis-trans isomerase Pin1(gene name synonym) uniprotkb:PIN1(gene name) psi-mi:PIN1(display_short) |
| Interaction detection methods | psi-mi:"MI:0077"(nuclear magnetic resonance) |
| First author | Zucchelli et al. (2013) |
| Identifier of the publication | pubmed:24267382 imex:IM-21758 |
| NCBI Taxonomy identifier for interactor A | taxid:9606(human) taxid:9606(Homo sapiens) |
| NCBI Taxonomy identifier for interactor B | taxid:9606(human) taxid:9606(Homo sapiens) |
| Interaction types | psi-mi:"MI:1237"(proline isomerization reaction) |
| Source databases and identifiers | psi-mi:"MI:0471"(MINT) |
| Interaction identifier(s) in the corresponding source database | intact:EBI-8830113 imex:IM-21758-1 |
| Confidence score | intact-miscore:0.61 |
| Complex expansion | - |
| Biological role A | Enzyme target |
| Biological role B | Enzyme |
| Experimental role A | Unspecified role |
| Experimental role B | Unspecified role |
| Interactor type A | Protein |
| Interactor type B | Protein |
| Annotations for the interaction | figure legend:f2a comment:"\"To this aim we first tested in vitro Pin1 enzyme activity on two peptides, EAERpTPWN and EAERTPWN, corresponding to Sp140-PHD L3 loop (Glu722-Asn729), with or without Threonine phosphorylation, respectively. Because of the slow exchange rate of the peptidyl prolyl cis-trans isomerization, several residues in both the free peptides displayed two distinct sets of 1H signals in 2D ROESY experiments (Fig. 2A-B). The cis and trans populations of both peptides were 15% and 85%, respectively, as estimated from the one-dimensional 1H and 2D 1H-13C HSQC spectra at room temperature. Exchange cross peaks were absent in the ROESY spectra of the free peptides, indicating that the exchange regime between the two conformations was too slow to be detected on the NMR timescale (Fig. 2A left, 2B left). Notably, addition of catalytic amounts of Pin1 to EAERpTPWN accelerated the isomerization rate of the phosphothreonine-prolyl bond, as shown by the appearance of exchange cross-peaks in the ROESY spectrum (Fig. 2A, right). As expected, in the presence of Pin1 no exchange peaks were observed for the non-phosphorylated control peptide (Fig. 2B, right).\"" curation depth:imex curation full coverage:Only protein-protein interactions |
| NCBI Taxonomy identifier for the host organism | taxid:-1(in vitro) taxid:-1(In vitro) |
| Parameters of the interaction | - |
| Creation date | 2013/10/28 |
| Update date | 2024/11/30 |
| negative Boolean value | false |
| Feature(s) for interactor A | phosphorylated residue:726-726 binding-associated region:722-729 |
| Feature(s) for interactor B | his tag:?-? |
| Stoichiometry for interactor A | - |
| Stoichiometry for interactor B | - |
| Participant identification method for interactor A | Predetermined participant |
| Participant identification method for interactor B | Predetermined participant |