| Unique identifier for interactor A | uniprotkb:Q9UPV9 |
| Unique identifier for interactor B | uniprotkb:Q12840 |
| Alternative identifier for interactor A | intact:EBI-1105048 uniprotkb:Q96B69 uniprotkb:Q63HR0 uniprotkb:E9PDS2 uniprotkb:Q659B5 uniprotkb:J3KNT7 ensembl:ENSP00000328998.5 |
| Alternative identifier for interactor B | intact:EBI-713468 uniprotkb:Q4LE26 uniprotkb:A6H8M5 ensembl:ENSP00000408979.2 |
| Aliases for A | psi-mi:trak1_human(display_long) uniprotkb:Protein Milton(gene name synonym) uniprotkb:TRAK1(gene name) psi-mi:TRAK1(display_short) uniprotkb:KIAA1042(gene name synonym) uniprotkb:OIP106(gene name synonym) uniprotkb:106 kDa O-GlcNAc transferase-interacting protein(gene name synonym) |
| Aliases for B | psi-mi:kif5a_human(display_long) uniprotkb:Neuronal kinesin heavy chain(gene name synonym) uniprotkb:Kinesin heavy chain neuron-specific 1(gene name synonym) uniprotkb:KIF5A(gene name) psi-mi:KIF5A(display_short) uniprotkb:NKHC1(gene name synonym) |
| Interaction detection methods | psi-mi:"MI:0007"(anti tag coimmunoprecipitation) |
| First author | Randall et al. (2013) |
| Identifier of the publication | pubmed:24161670 imex:IM-21706 |
| NCBI Taxonomy identifier for interactor A | taxid:9606(human) taxid:9606(Homo sapiens) |
| NCBI Taxonomy identifier for interactor B | taxid:9606(human) taxid:9606(Homo sapiens) |
| Interaction types | psi-mi:"MI:0915"(physical association) |
| Source databases and identifiers | psi-mi:"MI:0471"(MINT) |
| Interaction identifier(s) in the corresponding source database | intact:EBI-8786097 imex:IM-21706-4 |
| Confidence score | intact-miscore:0.56 |
| Complex expansion | - |
| Biological role A | Unspecified role |
| Biological role B | Unspecified role |
| Experimental role A | Bait |
| Experimental role B | Prey |
| Interactor type A | Protein |
| Interactor type B | Protein |
| Annotations for the interaction | figure legend:f5 comment:"\"Thus using this approach, three regions within the KIF5A Cterminal region were shown to contribute to the TRAK2 binding site i.e. KIF5A 942-961; KIF5A 877- 885 and KIF5A 861-877. (Note that full immunoblots for Figures 3 – 5 can be viewed in the Supplementary Information, Figures S1- S3). The region KIF5A 877-883 was studied in more detail. Three additional truncations were made at KIF5A 883, KIF5A 881 and KIF5A 879 plus the region 877- 883 was deleted. The constructs truncated at KIF5A 883, KIF5A 881 and KIF5A 879 co-immunoprecipitated with TRAK2 with a similar efficiency to KIF5A1-885 as indeed did the KIF5AΔ877-883 deletion. The latter was unexpected since this region, amino acid sequence GALKEAKE, had been clearly implicated in the efficiency of KIF5A/TRAK2 association (Figure 3D). It may be explained by an alteration of the KIF5A tertiary structure such that other amino acids requisite for the binding of TRAK2 are now available to compensate for the deleted region thus the predicted loss in efficacy of co-immunoprecipitation is circumvented. In the context of the full length KIF5A therefore, the other sites of interaction are sufficient to support partial association with TRAK2 even when 877-883 is removed.\"" curation depth:imex curation full coverage:Only protein-protein interactions |
| NCBI Taxonomy identifier for the host organism | taxid:9606(human-293a) taxid:9606(Homo sapiens HEK293A embryonic kidney cell) |
| Parameters of the interaction | - |
| Creation date | 2013/10/02 |
| Update date | 2024/11/30 |
| negative Boolean value | false |
| Feature(s) for interactor A | myc tag:?-? |
| Feature(s) for interactor B | his tag:?-? sufficient binding region:825-877 |
| Stoichiometry for interactor A | - |
| Stoichiometry for interactor B | - |
| Participant identification method for interactor A | Anti tag western blot |
| Participant identification method for interactor B | Anti tag western blot |