| Unique identifier for interactor A | uniprotkb:P50281 |
| Unique identifier for interactor B | uniprotkb:P16035 |
| Alternative identifier for interactor A | intact:EBI-992788 uniprotkb:Q6GSF3 uniprotkb:Q92678 uniprotkb:A8K5L0 ensembl:ENSP00000308208.6 |
| Alternative identifier for interactor B | intact:EBI-1033507 uniprotkb:Q16121 uniprotkb:Q93006 uniprotkb:Q9UDF7 ensembl:ENSP00000262768.6 |
| Aliases for A | psi-mi:mmp14_human(display_long) uniprotkb:Membrane-type matrix metalloproteinase 1(gene name synonym) uniprotkb:Membrane-type-1 matrix metalloproteinase(gene name synonym) uniprotkb:MMP-X1(gene name synonym) uniprotkb:MMP14(gene name) psi-mi:MMP14(display_short) |
| Aliases for B | psi-mi:timp2_human(display_long) uniprotkb:TIMP2(gene name) psi-mi:TIMP2(display_short) uniprotkb:Tissue inhibitor of metalloproteinases 2(gene name synonym) uniprotkb:CSC-21K(gene name synonym) |
| Interaction detection methods | psi-mi:"MI:0006"(anti bait coimmunoprecipitation) |
| First author | Niarakis et al. (2013) |
| Identifier of the publication | pubmed:24112707 imex:IM-21690 |
| NCBI Taxonomy identifier for interactor A | taxid:9606(human) taxid:9606(Homo sapiens) |
| NCBI Taxonomy identifier for interactor B | taxid:9606(human) taxid:9606(Homo sapiens) |
| Interaction types | psi-mi:"MI:0915"(physical association) |
| Source databases and identifiers | psi-mi:"MI:0471"(MINT) |
| Interaction identifier(s) in the corresponding source database | intact:EBI-8769407 imex:IM-21690-1 |
| Confidence score | intact-miscore:0.56 |
| Complex expansion | - |
| Biological role A | Unspecified role |
| Biological role B | Unspecified role |
| Experimental role A | Bait |
| Experimental role B | Prey |
| Interactor type A | Protein |
| Interactor type B | Protein |
| Annotations for the interaction | figure legend:f5a comment:"\"However, upon immunoprecipitation of sMT1-MMP from tissue extracts and fluids with polyclonal antibodies against the hinge region of MT1-MMP and western blot analysis, using monoclonal antibodies against human TIMP-2, an immunoreactive band of ~ 21 kDa, migrating as the human recombinant TIMP-2, was observed in all samples (Fig. 5A). Since polyclonal antibodies against the hinge region of MT1-MMP used for immunoprecipitation, do not recognize TIMP-2 (Fig. 5B), and they did not show non specific reaction with monoclonal antibodies against TIMP-2 (Fig. 5A), these results suggest that TIMP-2 co-precipitated with sMT1-MMP because it was already in complex with it. Consequently, it appears that the sMT1- MMP in tissue extracts and fluids exists in a latent form and yet, bound to TIMP-2. These results were further confirmed by reverse immunoprecipitation with monoclonal antibodies against TIMP-2 and western blotting using polyclonal antibodies against the hinge region of MT1-MMP. Only one immunoreactive band of ~ 57 kDa, corresponding to sMT1- MMP, was observed in all samples (Fig. 5C).\"" comment:"\"The same interaction has been detected in the following tissues: SF-OA, SF-RA, PSSF, POL, LUNG, PCT, IFT.\"" curation depth:imex curation full coverage:Only protein-protein interactions |
| NCBI Taxonomy identifier for the host organism | taxid:9606(human) taxid:9606(Homo sapiens) |
| Parameters of the interaction | - |
| Creation date | 2013/09/23 |
| Update date | 2024/11/14 |
| negative Boolean value | false |
| Feature(s) for interactor A | - |
| Feature(s) for interactor B | - |
| Stoichiometry for interactor A | - |
| Stoichiometry for interactor B | - |
| Participant identification method for interactor A | Predetermined participant |
| Participant identification method for interactor B | Western blot |