| Unique identifier for interactor A | uniprotkb:F1M7Y5 |
| Unique identifier for interactor B | uniprotkb:P19490 |
| Alternative identifier for interactor A | intact:EBI-8795211 uniprotkb:B1PZT8 ensembl:ENSRNOP00000012924.4 ensembl:ENSRNOP00055013108 ensembl:ENSRNOP00060002411 ensembl:ENSRNOP00065033924 |
| Alternative identifier for interactor B | intact:EBI-371642 ensembl:ENSRNOP00000064722.3 ensembl:ENSRNOP00055042480 ensembl:ENSRNOP00060032516 ensembl:ENSRNOP00065007367 |
| Aliases for A | psi-mi:kpci_rat(display_long) uniprotkb:Prkci(gene name) psi-mi:Prkci(display_short) uniprotkb:Pkcl(gene name synonym) uniprotkb:Atypical protein kinase C-lambda/iota(gene name synonym) uniprotkb:nPKC-iota(gene name synonym) |
| Aliases for B | psi-mi:gria1_rat(display_long) uniprotkb:Gria1(gene name) psi-mi:Gria1(display_short) uniprotkb:GluR-A(gene name synonym) uniprotkb:GluR-K1(gene name synonym) uniprotkb:Glutamate receptor ionotropic, AMPA 1(gene name synonym) uniprotkb:AMPA-selective glutamate receptor 1(gene name synonym) uniprotkb:Glur1(gene name synonym) uniprotkb:GluA1(gene name synonym) |
| Interaction detection methods | psi-mi:"MI:0006"(anti bait coimmunoprecipitation) |
| First author | Ren et al. (2013) |
| Identifier of the publication | pubmed:23511975 imex:IM-21577 |
| NCBI Taxonomy identifier for interactor A | taxid:10116(rat) taxid:10116("Rattus norvegicus (Rat)") |
| NCBI Taxonomy identifier for interactor B | taxid:10116(rat) taxid:10116("Rattus norvegicus (Rat)") |
| Interaction types | psi-mi:"MI:0914"(association) |
| Source databases and identifiers | psi-mi:"MI:0471"(MINT) |
| Interaction identifier(s) in the corresponding source database | intact:EBI-8795274 imex:IM-21577-3 |
| Confidence score | intact-miscore:0.35 |
| Complex expansion | psi-mi:"MI:1060"(spoke expansion) |
| Biological role A | Unspecified role |
| Biological role B | Unspecified role |
| Experimental role A | Bait |
| Experimental role B | Prey |
| Interactor type A | Protein |
| Interactor type B | Protein |
| Annotations for the interaction | figure legend:f4e f5c f5d sf14 comment:"\"Then, we employed anti-GluA1 antibody to precipitate the protein complex and to examine how PI3K activation would affect the association between these molecules. We found that upon 740Y-P treatment the amounts of p62 and PKCl that interacted with GluA1 (p62/GluA1 and PKCl/ GluA1) were significantly increased (Figures 4A and B), and the amount of P-Ser818-GluA1 was also increased simultaneously (Figures 4A and B).\"" comment:"\"According to this model, disrupting the interaction between p62 and GluA1 or between p62 and PKCl should hinder the formation of the trimeric PKCl/p62/GluA1 complex and in turn suppress GluA1 phosphorylation by PKCl. In order to test this hypothesis, we utilized short cell-permeable peptides derived from p62 binding sequence in the loop L2-3 of GluA1 [Tat-GluA1(L2-3)] or from the AID sequence of p62 (Tat-p62AID), to interfere with p62/GluA1 and PKCl/p62 interactions, respectively (Figures 5A and B). The efficacy and specificity of the peptides were evaluated by Co-IP assays. Tat-GluA1(L2-3) (2.0 mM) decreased the GluA1/ PKCl association (Figures 5C and E) but did not affect the p62/PKCl association (Figures 5C and E), whereas Tatp62AID (4.0 mM) significantly decreased both p62/PKCl and GluA1/PKCl association under basal condition (Figures 5C and E)\"" curation depth:imex curation full coverage:Only protein-protein interactions |
| NCBI Taxonomy identifier for the host organism | taxid:10116(rat-hippocampus) taxid:10116(Rattus norvegicus hippocampal neurons) |
| Parameters of the interaction | - |
| Creation date | 2013/10/15 |
| Update date | 2024/09/12 |
| negative Boolean value | false |
| Feature(s) for interactor A | - |
| Feature(s) for interactor B | - |
| Stoichiometry for interactor A | - |
| Stoichiometry for interactor B | - |
| Participant identification method for interactor A | Western blot |
| Participant identification method for interactor B | Western blot |