| Unique identifier for interactor A | uniprotkb:P10997 |
| Unique identifier for interactor B | uniprotkb:P10997 |
| Alternative identifier for interactor A | intact:EBI-8526679 uniprotkb:Q14598 intact:MINT-8074874 uniprotkb:Q0ZD87 ensembl:ENSP00000240652.3 ensembl:ENSP00000437357.1 |
| Alternative identifier for interactor B | intact:EBI-8526679 uniprotkb:Q14598 intact:MINT-8074874 uniprotkb:Q0ZD87 ensembl:ENSP00000240652.3 ensembl:ENSP00000437357.1 |
| Aliases for A | psi-mi:iapp_human(display_long) uniprotkb:IAPP(gene name) psi-mi:IAPP(display_short) uniprotkb:Amylin(gene name synonym) uniprotkb:Diabetes-associated peptide(gene name synonym) uniprotkb:Insulinoma amyloid peptide(gene name synonym) |
| Aliases for B | psi-mi:iapp_human(display_long) uniprotkb:IAPP(gene name) psi-mi:IAPP(display_short) uniprotkb:Amylin(gene name synonym) uniprotkb:Diabetes-associated peptide(gene name synonym) uniprotkb:Insulinoma amyloid peptide(gene name synonym) |
| Interaction detection methods | psi-mi:"MI:0051"(fluorescence technology) |
| First author | Peinado et al. (2013) |
| Identifier of the publication | pubmed:24042052 imex:IM-21426 |
| NCBI Taxonomy identifier for interactor A | taxid:9606(human) taxid:9606(Homo sapiens) |
| NCBI Taxonomy identifier for interactor B | taxid:9606(human) taxid:9606(Homo sapiens) |
| Interaction types | psi-mi:"MI:0407"(direct interaction) |
| Source databases and identifiers | psi-mi:"MI:0471"(MINT) |
| Interaction identifier(s) in the corresponding source database | intact:EBI-8755150 imex:IM-21426-1 |
| Confidence score | intact-miscore:0.95 |
| Complex expansion | - |
| Biological role A | Unspecified role |
| Biological role B | Unspecified role |
| Experimental role A | Unspecified role |
| Experimental role B | Unspecified role |
| Interactor type A | Protein |
| Interactor type B | Protein |
| Annotations for the interaction | figure legend:f1 f2 f3 comment:"\"We investigated hIAPP fibrillation using an in vitro fluorescence assay using a ThT assay. In our hands, concentrations ranging from 5 to 20 μM of hIAPP exhibited complete fibrillation within an hour at 25 C and 10 μM was chosen for further assays (Fig. 1A). It has been shown that fibrillation of hIAPP is inhibited by insulin [25]. In our assays 5 μM insulin blocked 80% of hIAPP fibrillation, while an irrelevant control protein such as carbonic anhydrase did not block fibrillation (Fig. 1B).\"" comment:"\"We found 90% inhibition of hIAPP fibrillation with 10 μM 7B2, while 1 μM resulted in 50% inhibition (Fig. 1C). ProSAAS is also abundant in the pancreas [27]; in order to evaluate whether proSAAS also possesses anti-fibrillation ability, we carried out an assay using proSAAS concentrations between 0.1 and 10 μM. Under these conditions 10 μM 21 kDa proSAAS blocked hIAPP fibrillation completely and 0.1 μM still retained some inhibitory activity on fibril formation (Fig. 1D).\"" comment:"\"To elucidate the region within 7B2 responsible for the anti-fibrillation effect, we performed in vitro fibrillation assays with N-terminally truncated proteins. Structure-function analysis using truncated forms of 7B2 revealed that the anti-aggregation effect was greatest using 21-kDa 7B2 (Fig. 2A), although 10 μM 7B230-150 and 7B268-150 partially inhibited hIAPP fibrillation by 30 and 20% respectively (Fig. 2B).\"" comment:"\"two truncated peptides (Fig. 3A) were synthesized (proSAAS97-137 and proSAAS138-180, see Materials and Methods) to test whether α-helices II and III play a role in proSAAS-induced inhibition of fibrillation. However, neither of these two peptides was able to inhibit fibrillation in vitro (Fig. 3B). We conclude that residues 1-97 in the N-terminal domain contain key determinants for the anti-fibrillation ability of proSAAS on hIAPP\"" full coverage:Only protein-protein interactions curation depth:imex curation |
| NCBI Taxonomy identifier for the host organism | taxid:-1(in vitro) taxid:-1(In vitro) |
| Parameters of the interaction | - |
| Creation date | 2013/09/06 |
| Update date | 2014/10/16 |
| negative Boolean value | false |
| Feature(s) for interactor A | - |
| Feature(s) for interactor B | - |
| Stoichiometry for interactor A | - |
| Stoichiometry for interactor B | - |
| Participant identification method for interactor A | Predetermined participant |
| Participant identification method for interactor B | Predetermined participant |