| Unique identifier for interactor A | uniprotkb:Q13509 |
| Unique identifier for interactor B | uniprotkb:P68363 |
| Alternative identifier for interactor A | intact:EBI-350989 ensembl:ENSP00000320295.7 uniprotkb:A8K854 uniprotkb:Q9BTZ0 uniprotkb:Q9BW10 |
| Alternative identifier for interactor B | intact:EBI-487083 uniprotkb:Q8WU19 ensembl:ENSP00000336799.5 intact:EBI-1384226 uniprotkb:P04687 uniprotkb:P05209 uniprotkb:Q27I68 |
| Aliases for A | psi-mi:tbb3_human(display_long) uniprotkb:TUBB3(gene name) psi-mi:TUBB3(display_short) uniprotkb:TUBB4(gene name synonym) uniprotkb:Tubulin beta-III(gene name synonym) uniprotkb:Tubulin beta-4 chain(gene name synonym) |
| Aliases for B | psi-mi:tba1b_human(display_long) uniprotkb:TUBA1B(gene name) psi-mi:TUBA1B(display_short) uniprotkb:Tubulin alpha-ubiquitous chain(gene name synonym) uniprotkb:Alpha-tubulin ubiquitous(gene name synonym) uniprotkb:Tubulin K-alpha-1(gene name synonym) |
| Interaction detection methods | psi-mi:"MI:0004"(affinity chromatography technology) |
| First author | Minoura et al. (2013) |
| Identifier of the publication | pubmed:24021646 imex:IM-21411 |
| NCBI Taxonomy identifier for interactor A | taxid:9606(human) taxid:9606(Homo sapiens) |
| NCBI Taxonomy identifier for interactor B | taxid:9606(human) taxid:9606(Homo sapiens) |
| Interaction types | psi-mi:"MI:0914"(association) |
| Source databases and identifiers | psi-mi:"MI:0471"(MINT) |
| Interaction identifier(s) in the corresponding source database | intact:EBI-8716172 imex:IM-21411-1 |
| Confidence score | intact-miscore:0.56 |
| Complex expansion | - |
| Biological role A | Unspecified role |
| Biological role B | Unspecified role |
| Experimental role A | Unspecified role |
| Experimental role B | Unspecified role |
| Interactor type A | Protein |
| Interactor type B | Protein |
| Annotations for the interaction | figure legend:f2 f3b f3c comment:"\"The presence of both endogenous and recombinant alpha-tubulin in the eluent from the His-affinity column indicates that His-tagged alpha1-tubulin can form dimers with either type of alpha-tubulin (Fig. 2D). Vice versa, when DEAE eluent was first applied to the FLAG-affinity column instead of the Hisaffinity column, the eluent from the FLAG-affinity column contained both endogenous and recombinant alpha±-tubulin, indicating that alpha3-tubulin can form dimers with either type of alpha±-tubulin (data not shown). Because recombinant tubulin forms dimers with its endogenous counterpart, certain fractions of soluble alpha1- and alpha3-polypeptides originally expressed in the HighFive cells (Fig. 1B) were inevitably lost during the purification process (Fig. 2D).\"" full coverage:Only protein-protein interactions curation depth:imex curation |
| NCBI Taxonomy identifier for the host organism | taxid:-1(in vitro) taxid:-1(In vitro) |
| Parameters of the interaction | - |
| Creation date | 2013/08/29 |
| Update date | 2014/10/16 |
| negative Boolean value | false |
| Feature(s) for interactor A | flag tag:c-c |
| Feature(s) for interactor B | his tag:c-c |
| Stoichiometry for interactor A | - |
| Stoichiometry for interactor B | - |
| Participant identification method for interactor A | De novo protein sequencing by mass spectrometry |
| Participant identification method for interactor B | De novo protein sequencing by mass spectrometry |