Curation Details
Interaction ID: IM-21408-3

Unique identifier for interactor Auniprotkb:P33991
Unique identifier for interactor Buniprotkb:Q9BTE3
Alternative identifier for interactor Aintact:EBI-374938
uniprotkb:Q8NEH1
uniprotkb:Q99658
ensembl:ENSP00000262105.2
ensembl:ENSP00000496964.1
Alternative identifier for interactor Bintact:EBI-749378
uniprotkb:Q6IA56
uniprotkb:Q9BVT9
uniprotkb:Q9H916
uniprotkb:B3KSP7
ensembl:ENSP00000353098.3
Aliases for Apsi-mi:mcm4_human(display_long)
uniprotkb:MCM4(gene name)
psi-mi:MCM4(display_short)
uniprotkb:CDC21(gene name synonym)
uniprotkb:CDC21 homolog(gene name synonym)
uniprotkb:P1-CDC21(gene name synonym)
Aliases for Bpsi-mi:mcmbp_human(display_long)
uniprotkb:MCMBP(gene name)
psi-mi:MCMBP(display_short)
uniprotkb:C10orf119(gene name synonym)
Interaction detection methodspsi-mi:"MI:0006"(anti bait coimmunoprecipitation)
First authorNguyen et al. (2012)
Identifier of the publicationpubmed:22540012
imex:IM-21408
NCBI Taxonomy identifier for interactor Ataxid:9606(human)
taxid:9606(Homo sapiens)
NCBI Taxonomy identifier for interactor Btaxid:9606(human)
taxid:9606(Homo sapiens)
Interaction typespsi-mi:"MI:0915"(physical association)
Source databases and identifierspsi-mi:"MI:0471"(MINT)
Interaction identifier(s) in the corresponding source databaseintact:EBI-8756064
imex:IM-21408-3
Confidence scoreintact-miscore:0.85
Complex expansion-
Biological role AUnspecified role
Biological role BUnspecified role
Experimental role APrey
Experimental role BBait
Interactor type AProtein
Interactor type BProtein
Annotations for the interactionfigure legend:sf1
comment:These results indicate that all MCM2-8 proteins can interact with MCM-BP
comment:"\"To examine the interaction of human MCM-BP with each subunit of MCM2-7, each subunit was co-expressed with MCM-BP in insect cells, and then, MCM-BP in the cell lysate was immuno-precipitated with anti-MCM-BP antibody beads. As a control, cell lysate expressing the MCM subunit only was immuno-precipitated with anti-MCM-BP antibody beads. In addition to proteins in the precipitated fraction (eluate), proteins in the cell lysate (sup), those in Triton-insoluble fraction (ppt), those in the supernatant after incubation with antibody beads (ub) and those in the supernatant of the final wash (wash) were examined by western-blot analysis (supplementary Fig. 1A).\""
curation depth:imex curation
full coverage:Only protein-protein interactions
NCBI Taxonomy identifier for the host organismtaxid:7108(spofr)
taxid:7108("Spodoptera frugiperda (Fall armyworm)")
Parameters of the interaction-
Creation date2013/09/09
Update date2024/11/22
negative Boolean valuefalse
Feature(s) for interactor A-
Feature(s) for interactor B-
Stoichiometry for interactor A-
Stoichiometry for interactor B-
Participant identification method for interactor AWestern blot
Participant identification method for interactor BWestern blot