Curation Details
Interaction ID: IM-21408-19

Unique identifier for interactor Auniprotkb:O00311
Unique identifier for interactor Buniprotkb:Q9BTE3
Alternative identifier for interactor Aintact:EBI-374980
uniprotkb:D3DT31
ensembl:ENSP00000234626.6
ensembl:ENSP00000393139.1
uniprotkb:O00558
uniprotkb:Q5T5U5
Alternative identifier for interactor Bintact:EBI-749378
uniprotkb:B3KSP7
ensembl:ENSP00000353098.3
uniprotkb:Q6IA56
uniprotkb:Q9BVT9
uniprotkb:Q9H916
Aliases for Apsi-mi:cdc7_human(display_long)
uniprotkb:CDC7(gene name)
psi-mi:CDC7(display_short)
uniprotkb:CDC7L1(gene name synonym)
Aliases for Bpsi-mi:mcmbp_human(display_long)
uniprotkb:MCMBP(gene name)
psi-mi:MCMBP(display_short)
uniprotkb:C10orf119(gene name synonym)
Interaction detection methodspsi-mi:"MI:0006"(anti bait coimmunoprecipitation)
First authorNguyen et al. (2012)
Identifier of the publicationpubmed:22540012
imex:IM-21408
NCBI Taxonomy identifier for interactor Ataxid:9606(human)
taxid:9606(Homo sapiens)
NCBI Taxonomy identifier for interactor Btaxid:9606(human)
taxid:9606(Homo sapiens)
Interaction typespsi-mi:"MI:0915"(physical association)
Source databases and identifierspsi-mi:"MI:0471"(MINT)
Interaction identifier(s) in the corresponding source databaseintact:EBI-8759569
imex:IM-21408-19
Confidence scoreintact-miscore:0.59
Complex expansion-
Biological role AUnspecified role
Biological role BUnspecified role
Experimental role APrey
Experimental role BBait
Interactor type AProtein
Interactor type BProtein
Annotations for the interactionfigure legend:sf1
comment:These results indicate that all MCM2-8 proteins can interact with MCM-BP
comment:"\"To examine the interaction of human MCM-BP with each subunit of MCM2-7, each subunit was co-expressed with MCM-BP in insect cells, and then, MCM-BP in the cell lysate was immuno-precipitated with anti-MCM-BP antibody beads. As a control, cell lysate expressing the MCM subunit only was immuno-precipitated with anti-MCM-BP antibody beads. In addition to proteins in the precipitated fraction (eluate), proteins in the cell lysate (sup), those in Triton-insoluble fraction (ppt), those in the supernatant after incubation with antibody beads (ub) and those in the supernatant of the final wash (wash) were examined by western-blot analysis (supplementary Fig. 1A).\""
full coverage:Only protein-protein interactions
curation depth:imex curation
NCBI Taxonomy identifier for the host organismtaxid:7108(spofr)
taxid:7108("Spodoptera frugiperda (Fall armyworm)")
Parameters of the interaction-
Creation date2013/09/09
Update date2014/10/16
negative Boolean valuefalse
Feature(s) for interactor Aha tag:?-?
Feature(s) for interactor B-
Stoichiometry for interactor A-
Stoichiometry for interactor B-
Participant identification method for interactor AWestern blot
Participant identification method for interactor BWestern blot