| Unique identifier for interactor A | uniprotkb:Q9BTE3 |
| Unique identifier for interactor B | uniprotkb:P25205 |
| Alternative identifier for interactor A | intact:EBI-749378 uniprotkb:B3KSP7 ensembl:ENSP00000353098.3 uniprotkb:Q6IA56 uniprotkb:Q9BVT9 uniprotkb:Q9H916 |
| Alternative identifier for interactor B | intact:EBI-355153 ensembl:ENSP00000472940.2 uniprotkb:B4DWW4 uniprotkb:Q92660 uniprotkb:Q9BTR3 uniprotkb:Q9NUE7 |
| Aliases for A | psi-mi:mcmbp_human(display_long) uniprotkb:MCMBP(gene name) psi-mi:MCMBP(display_short) uniprotkb:C10orf119(gene name synonym) |
| Aliases for B | psi-mi:mcm3_human(display_long) uniprotkb:MCM3(gene name) psi-mi:MCM3(display_short) uniprotkb:DNA polymerase alpha holoenzyme-associated protein P1(gene name synonym) uniprotkb:RLF subunit beta(gene name synonym) uniprotkb:p102(gene name synonym) uniprotkb:P1-MCM3(gene name synonym) |
| Interaction detection methods | psi-mi:"MI:0096"(pull down) |
| First author | Nguyen et al. (2012) |
| Identifier of the publication | pubmed:22540012 imex:IM-21408 |
| NCBI Taxonomy identifier for interactor A | taxid:9606(human) taxid:9606(Homo sapiens) |
| NCBI Taxonomy identifier for interactor B | taxid:9606(human) taxid:9606(Homo sapiens) |
| Interaction types | psi-mi:"MI:0914"(association) |
| Source databases and identifiers | psi-mi:"MI:0471"(MINT) |
| Interaction identifier(s) in the corresponding source database | intact:EBI-8756410 imex:IM-21408-18 |
| Confidence score | intact-miscore:0.85 |
| Complex expansion | psi-mi:"MI:1060"(spoke expansion) |
| Biological role A | Unspecified role |
| Biological role B | Unspecified role |
| Experimental role A | Bait |
| Experimental role B | Prey |
| Interactor type A | Protein |
| Interactor type B | Protein |
| Annotations for the interaction | figure legend:f5 comment:"\"To understand the interaction of MCM2-7 with MCM-BP at protein level, following experiment was carried out. Flag-tagged MCM-BP was co-expressed with MCM2-7 proteins in insect cells. Flag-tagged MCM-BP was purified using anti-Flag-agarose beads, and co-purified MCM2-7 proteins were analyzed (Fig. 5). Purified protein complex containing Flag-MCM-BP was separated by glycerol gradient centrifugation. Western-blot analysis where purified MCM2-7 proteins were co-electrophoresed shows that MCM3, MCM5 and MCM7 were mainly recovered with MCM-BP and smaller amounts of MCM4 and MCM6 were also recovered, suggesting that MCM-BP has higher affinity for MCM3, MCM5 and MCM7. Position of sedimentation suggests that MCM-BP mainly form dimmers by interacting with MCM3, MCM5 or MCM7. Thus these all results suggest that MCM-BP disassembles MCM2-7 complex on the DNA by interacting with MCM3, MCM5 and MCM7 proteins.\"" full coverage:Only protein-protein interactions curation depth:imex curation |
| NCBI Taxonomy identifier for the host organism | taxid:7108(spofr) taxid:7108("Spodoptera frugiperda (Fall armyworm)") |
| Parameters of the interaction | - |
| Creation date | 2013/09/09 |
| Update date | 2014/10/16 |
| negative Boolean value | false |
| Feature(s) for interactor A | flag tag:?-? |
| Feature(s) for interactor B | - |
| Stoichiometry for interactor A | - |
| Stoichiometry for interactor B | - |
| Participant identification method for interactor A | Western blot |
| Participant identification method for interactor B | Western blot |