| Unique identifier for interactor A | uniprotkb:Q9QWY8 |
| Unique identifier for interactor B | uniprotkb:P84080 |
| Alternative identifier for interactor A | intact:EBI-698498 uniprotkb:Q99LV8 uniprotkb:Q9Z2B6 uniprotkb:O08612 uniprotkb:Q80TG8 uniprotkb:Q80UV6 uniprotkb:Q505F0 ensembl:ENSMUSP00000134825.2 |
| Alternative identifier for interactor B | intact:EBI-449051 uniprotkb:A5D7F1 uniprotkb:P10947 uniprotkb:P32889 uniprotkb:Q3SZG1 ensembl:ENSBTAP00000010159.4 |
| Aliases for A | psi-mi:asap1_mouse(display_long) uniprotkb:PIP2-dependent ARF1 GAP(gene name synonym) uniprotkb:ADP-ribosylation factor-directed GTPase-activating protein 1(gene name synonym) uniprotkb:Development and differentiation-enhancing factor 1(gene name synonym) uniprotkb:Asap1(gene name) psi-mi:Asap1(display_short) uniprotkb:Ddef1(gene name synonym) uniprotkb:Kiaa1249(gene name synonym) uniprotkb:Shag1(gene name synonym) uniprotkb:130 kDa phosphatidylinositol 4,5-bisphosphate-dependent ARF1 GTPase-activating protein(gene name synonym) |
| Aliases for B | psi-mi:arf1_bovin(display_long) uniprotkb:ARF1(gene name) psi-mi:ARF1(display_short) |
| Interaction detection methods | psi-mi:"MI:0027"(cosedimentation) |
| First author | Klein et al. (2005) |
| Identifier of the publication | pubmed:16223492 mint:MINT-5218002 imex:IM-11058 |
| NCBI Taxonomy identifier for interactor A | taxid:10090(mouse) taxid:10090(Mus musculus) |
| NCBI Taxonomy identifier for interactor B | taxid:9913(bovin) taxid:9913("Bos taurus (Bovine)") |
| Interaction types | psi-mi:"MI:0407"(direct interaction) |
| Source databases and identifiers | psi-mi:"MI:0471"(MINT) |
| Interaction identifier(s) in the corresponding source database | intact:EBI-698541 imex:IM-11058-1 |
| Confidence score | intact-miscore:0.56 |
| Complex expansion | - |
| Biological role A | Unspecified role |
| Biological role B | Unspecified role |
| Experimental role A | Neutral component |
| Experimental role B | Neutral component |
| Interactor type A | Protein |
| Interactor type B | Protein |
| Annotations for the interaction | comment:"\"liposome sedimentation: only 27% of myr-Arf1 GDP and 34% of ASAP1 PZA fragment were recovered in the pelleted membrane fraction if alone. The addition of AlFx did not change the sedimentation properties of each proteins. When mixed together in presence of AlFx, both PZA and and myr-Arf1 translocated to membrane fraction within the same proportion 74% and 83% respectively.\"" stimulant:"\"AlFx (Aluminum fluoride) stabilize the complex formation between Arf1-GDP and PZA fragment of ASAP1 by mimicking activated form of Arf1, Arf1-GDP-AlFx.\"" comment:Both proteins are purified to homogeneity. Myristoylated Arf1 was produced by co-expressing in E. coli the yeast N-myristoyl-transferase. curation depth:imex curation full coverage:Only protein-protein interactions |
| NCBI Taxonomy identifier for the host organism | taxid:-1(in vitro) taxid:-1(In vitro) |
| Parameters of the interaction | - |
| Creation date | 2005/10/17 |
| Update date | 2025/07/17 |
| negative Boolean value | false |
| Feature(s) for interactor A | sufficient binding region:325-724 his tag:n-n |
| Feature(s) for interactor B | N-myristoylglycine:2-2 |
| Stoichiometry for interactor A | - |
| Stoichiometry for interactor B | - |
| Participant identification method for interactor A | Predetermined participant |
| Participant identification method for interactor B | Predetermined participant |